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Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDP085
Plasmid#198425PurposepUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120DepositorInsertE. coli BirA
UseTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TS_pLuxLac
Plasmid#140426PurposeToggle switch circuit responsive to AHL gradient.DepositorInsertsGFP
LacI
TetR
mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-LDLR-mCherry BlastR
Plasmid#186739PurposeDox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertLDLR (LDLR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-AcKRS
Plasmid#140021PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC19-13XLexAop2-IVS-myr::GFP
Plasmid#26224DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-tubulin
Plasmid#24327DepositorInsertTubulin (TUBA1B Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
DiLiCre 2.0
Plasmid#198752PurposeA Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertCre-PhoCI-ERT2-ERT2
UseLentiviralTagsCre, kept in a photocleavable site, followed by 2…ExpressionBacterial and MammalianMutationPromoterTRE3GVAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiV2-dCas9-VP64
Plasmid#141104PurposeExpression of gRNA and dCas9-VP64 from the same plasmid for CRISPRaDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG
Plasmid#138566PurposeExpresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationL1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_JN.1
Plasmid#218678Purposeexpressing SARS-CoV-2 JN.1 spike protein for pseudovirus productionDepositorInsertSpike (JN.1)
UseTagsExpressionMammalianMutationL455S on the basis of BA.2.86PromoterCMVAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KIAA1549_BRAF
Plasmid#205838PurposeExpress mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAD-F3-NPEST-iCRE-2A-mCherryPEST
Plasmid#99608PurposeSelf-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherryDepositorInsertsiCRE
mCherry
UseUnspecifiedTagsPESTExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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MCP-M-MLV RT∆RNase H
Plasmid#213749PurposeFor circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cellsDepositorInsertMCP-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pCDNA3
Plasmid#107173Purposeexpresses V5-tagged TurboID on the mammalian ER membraneDepositorInsertTurboID (BirA mutant)
UseTagsExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV296
Plasmid#204687PurposeAMA1 plasmid with nat1 resistance markerDepositorInsertnat1
UseFungal expressionTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73037PurposeExpress Nluc and copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEGFP
Plasmid#18696DepositorInsertmonomeric EGFP
UseTagsExpressionMammalianMutationA206K mutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only