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Plasmid#25066DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationContains only the ROC-COR-Kinase domains aa 1329…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
P38-bio
Plasmid#47727PurposeExpresses enzymatically monobiotinylated full-length P38 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised P38
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pM-ErbB4CTF-PY3 mutant
Plasmid#17799DepositorInsertErbB4 (ERBB4 Human)
UseTagsGAL4-BDExpressionMammalianMutationCarboxy-terminal fragment, amino acids 676-1292, …PromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf144
Plasmid#12832PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf144
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOCC177
Plasmid#118886Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease and SNAP tag…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC194
Plasmid#118882Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
UseTagsSNAP tag, cleavable with TEV protease, HIS6, clea…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC231
Plasmid#118885Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease and mCitrine…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
VP16-hAR
Plasmid#89092Purposemammalian expression of full-length human androgen receptor with added N-terminal VP16 activation domainDepositorInserthAR (AR Human)
UseTagsVP16ExpressionMammalianMutationPromoterSV40Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.a(L)-Fc-His
Plasmid#72151PurposeExpresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3g.a (Sema3g Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
ROZA-XL YF
Plasmid#64195PurposeFluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequenceDepositorInsertROZA-XL YF
UseTagsExpressionMammalianMutationROZA-XL tyrosine phosphorylation site mutated to …PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-xlH2B(1-114)_K114A
Plasmid#118217PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.DepositorInsertxlH2B(1-114)
UseTagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialMutationLysine 114 to alaninePromoterT7Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.4-Fc-His
Plasmid#72170PurposeExpresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6d.4 (Sema6d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)
Plasmid#86818Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
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MSP11-bio
Plasmid#47734PurposeExpresses enzymatically monobiotinylated full-length MSP11 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP11
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSRP1-bio
Plasmid#47736PurposeExpresses enzymatically monobiotinylated full-length MSRP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSRP1
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSRP2-bio
Plasmid#47737PurposeExpresses enzymatically monobiotinylated full-length MSRP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSRP2
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSRP3-bio
Plasmid#47738PurposeExpresses enzymatically monobiotinylated full-length MSRP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSRP3
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
AARP-bio
Plasmid#47783PurposeExpresses enzymatically monobiotinylated full-length AARP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised AARP
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pf34-bio
Plasmid#47784PurposeExpresses enzymatically monobiotinylated full-length Pf34 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised Pf34
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only