ROZA-XL YF
(Plasmid #64195)

• Purpose: Fluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequence
• Depositing Lab: Annemarie Lellouch
• Publication: Cadra et al Biochem Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5. doi: 10.1016/j.bbrc.2015.02.117.

Backbone

• Vector backbone: pCDNA 3.1+
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5445
• Total vector size (bp): 1846
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ROZA-XL YF
• Species: Synthetic
• Mutation: ROZA-XL tyrosine phosphorylation site mutated to phenylalanine
• GenBank ID: KM979358
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This construct includes a YFP varient "YPET" that I obtained from PS Daugherty, UC Davis, USA (see reference below) and a monomeric variant of CFP that was obtained from RY Tsien UC San Diego, USA (see reference below)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference for CFP:
Zacharias DA, Violin JD, Newton AC, Tsien RY (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296: 913-916.

Reference for YPET, yellow fluorescent protein:
A.W. Nguyen, P.S. Daugherty, Evolutionary optimization of fluorescent proteins for intracellular FRET, Nat. Biotechnol. 23 (2005) 355e360