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Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
UseTagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ
Plasmid#64875Purposelentiviral expression of human POLQDepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationPromoterEF1alphaAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p58sheLL
Plasmid#37324DepositorInsertHPV58L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a-His-IleRS
Plasmid#124112PurposeBacterial expression plasmid for PURE system componentsDepositorInsertIsoleucyl-tRNA ligase (ileS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX317-MBNL1
Plasmid#115443PurposeConstitutive lentiviral expressionDepositorInsertMBNL1 (MBNL1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Nanog
Plasmid#13354PurposeRetroviral expression of mouse NanogDepositorInsertNanog homeobox (Nanog Mouse)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP21
Plasmid#22574DepositorInsertUSP21 (USP21 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHes7-Achilles-Hes7
Plasmid#153528PurposeExpresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.DepositorInsertHes7 (Hes7 Mouse)
UseTagsAchillesExpressionMammalianMutationPromotermouse Hes7 promoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
UseTagsFis1ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
GW1-Mito-pHRed
Plasmid#31474DepositorInsertCox8-tagged pHRed
UseTags4xCox8ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCSCMV:tdTomato
Plasmid#30530Purposemammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.DepositorHas ServiceCloning Grade DNAInserttdTomato
UseTagsExpressionMammalianMutationR415HPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAD48-daf-2 RNAi
Plasmid#34834DepositorInsertdaf-2 (daf-2 Nematode)
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-TIS11B
Plasmid#121157PurposeMammalian expression of GFP tagged TIS11BDepositorInsertZFP36L1 (TIS11B) (ZFP36L1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry
Plasmid#197420PurposeCas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNADepositorInsertCas9
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-V5-TurboID(N)-GPI
Plasmid#163696PurposeExpresses n-terminal half of TurboID on surface of neuronsDepositorInsertGPI anchored TurboID
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-ICAM1
Plasmid#156462PurposeExpresses a rabbit Fc-ICAM1 fusion protein in mammalian cellsDepositorInsertIntercellular Adhesion Molecule 1 (Icam1 Mouse)
UseTagsICAM1-rFcExpressionBacterial and MammalianMutationendogenous signal peptide replaced with IL2 signa…PromoterhEF1-HTLCAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD162 (Peft-3::Cas9 + Empty sgRNA)
Plasmid#47549PurposeCas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.DepositorInsertsCas9
Empty sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFR_HCV_xb
Plasmid#11510DepositorInsertFirefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only