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We narrowed to 15,902 results for: grna

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Showing: 7241 - 7260 of 15902 results

  6. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid
    #120250
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    120250-plasmid-map-sequence-id-238538

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  11. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid
    #184840
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Publication
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184840-plasmid-map-sequence-id-373961

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  16. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid
    #190112
    Purpose
    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.
    Inserts
    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    Use
    AAV
    Tags
    bpNLS and bpNLS-P2A-eGFP
    Mutation
    mutations from RT in PE2 and truncation of RNAse …
    Promoter
    EFS and U6, H1
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    190112-plasmid-map-sequence-id-372717

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  17. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    181745-plasmid-map-sequence-id-354676

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Showing: 7241 - 7260 of 15902 results