Addgene

Showing: 3621 - 3630 of 9036 results

Results per page:

Sort results by:

pAcBac1-EcTyr-TAG

Plasmid

#228782

Purpose

EcTyr aaRS/tRNA system for mammalian ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
BstR

Use

Baculoviral

Tags

Expression

Mammalian

Mutation

TAG suppressor

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR1A 2xHA GPR137

Plasmid

#226809

Purpose

Gateway entry vector for GPR137

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Expression

Mutation

Sequence optimized from NP_001164351

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-GST-BcPC-PLC

Plasmid

#224255

Purpose

Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

Depositor

Chiaki Murakami

Article

Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

Insert

Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)

Use

Tags

GST and HRV-3C (LEVLFQGP) site

Expression

Bacterial

Mutation

The gene of Bacillus cereus ATCC 14579 was used f…

Promoter

lac promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJExpress401-KDAC4 CD H976Y

Plasmid

#224250

Purpose

Expresses human KDAC4 (HDAC4) catalytic domain H976Y in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2017 Aug 29;56(34):4549-45

Insert

KDAC4 (HDAC4 Human)

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 648-1083 (catalytic domain…

Promoter

T5

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFastBacI-KDAC6 H216A

Plasmid

#224351

Purpose

Expresses human KDAC6 (HDAC6) H216A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 216 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFastBacI-KDAC6 H611A

Plasmid

#224352

Purpose

Expresses human KDAC6 (HDAC6) H611A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 611 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-MCHpr-DREADD (Gq)-mCherry

Plasmid

#221036

Purpose

Expresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Wang et al Mol Psychiatry. 2021 Jul;26(7):3152-3168

Insert

DREADD Gq-mCherry

Use

AAV; Rat targeting

Tags

Expression

Mammalian

Mutation

Promoter

Rat MCH

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-TAF5-FL_WobbleMut

Plasmid

#209053

Purpose

Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-TAF5-∆ex8_WobbleMut

Plasmid

#209054

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
H6GB1tev-IntC-P53(304-393)

Plasmid

#200315

Purpose

bacterial expression of P53 TETCTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Use

Tags

His6GB1tev and designed intein C

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge