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Showing: 7221 - 7240 of 22202 results

  4. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  5. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  8. pcDNA3.2-GJA1-20K-V5

    Plasmid
    #49859
    Purpose
    Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    GJA1-20k (GJA1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    GJA1-20k fragment corresponds to residues 213-382…
    Promoter
    CMV
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid
    #157716
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157716-plasmid-map-sequence-id-307912

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  12. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid
    #157699
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157699-plasmid-map-sequence-id-307863

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  13. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid
    #157693
    Purpose
    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157693-plasmid-map-sequence-id-307835

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  16. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  17. pCF806-shABC2

    Plasmid
    #186714
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    186714-plasmid-map-sequence-id-382420

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  18. pCF806-shABC1

    Plasmid
    #186713
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    186713-plasmid-map-sequence-id-376087

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  19. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Plasmid
    #217014
    Purpose
    Expression of CDK2-7 chimera in E.coli
    Depositor
    Manjeet Mukherjee
    Article
    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018
    Insert
    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
    Use
    Tags
    C3 protease cleavable GST tag
    Expression
    Bacterial
    Mutation
    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217014-plasmid-map-sequence-id-431050

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Showing: 7221 - 7240 of 22202 results