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Plasmid#107448PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet26 (met26 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet16
Plasmid#107452PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet16 (met16 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PKM2
Plasmid#34608DepositorInsertPKM2 (PKM Human)
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
GGE152
Plasmid#120752PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_8UASxpr-TEF)DepositorInsertP2_8UASxpr-TEF
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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YIPlac204TKC-GFP-HDEL
Plasmid#21771DepositorInsertGreen Fluorescent Protein (GFP)
UseTagsHDEL and preKar2ExpressionYeastMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
GGE151
Plasmid#120751PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_4UASxpr-TEF)DepositorInsertP2_4UASxpr-TEF
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-Phleomycin
Plasmid#188930PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-Phleomycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet8
Plasmid#107449PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet8
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSua1
Plasmid#107446PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSua1
UseTagsV5-HisExpressionYeastMutationSee depositor comments below.PromoterGAL1Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet14
Plasmid#107451PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet14 (met14 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet1
Plasmid#107444PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet1
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Kan
Plasmid#188918PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Kan
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GGE250
Plasmid#120746PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-5AB)DepositorInsertP1_EYK300-5AB
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE150
Plasmid#120750PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_2UASxpr-TEF)DepositorInsertP2_2UASxpr-TEF
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen (pKBJ010)
Plasmid#173461PurposeC-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8-cerulean3
Plasmid#99358PurposeBacterial expression of Cerulean3 fused to the 3' of cDNA of the fission yeast tropomyosin, cdc8.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsCerulean3 and Gly3-His6ExpressionBacterialMutationPromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYM28-BirA*
Plasmid#160290PurposeUsed for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: HIS3DepositorInsertBirA*
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-G76V-GFP
Plasmid#11954DepositorInsertUb-G76V-GFP
UseTagsExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …PromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only