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pLL3.7-K122-TEAD-p-H2B-mCherry

Plasmid

#139648

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

IRES-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

Plasmid

#199364

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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10XSTAT92E–luciferase

Plasmid

#37393

Depositor

Norbert Perrimon

Article

Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

Insert

SOCS36E enhancer fragment (Socs36E Fly)

Use

Luciferase; Jak/stat reporter construct

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV Puro SCRIB WT

Plasmid

#88886

Purpose

SCRIB WT in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Tags

N/A

Expression

Mutation

N/A

Promoter

MSCV promoter

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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P447-FBP_19

Plasmid

#139710

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP Flag-hRpt6

Plasmid

#19793

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

hRpt6 (PSMC5 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-HA-human CMYC (208-439)

Plasmid

#203433

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Use

Tags

2 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SHIP2-SH2

Plasmid

#46493

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP2

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 8-131

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1 CA

Plasmid

#79502

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Multisite gateway entry clone

Tags

3XFLAG

Expression

Mutation

S127A

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inpp5e(D477N)

Plasmid

#96809

Purpose

phosphatase-dead control for 5HT6-YFP-Inpp5e, CAAX-null

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inpp5e(D477N)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pBS hBAF53a

Plasmid

#17879

Depositor

Jerry Crabtree

Article

Zhao et al Cell. 1998 Nov 25. 95(5):625-36.

Insert

BAF53a (ACTL6A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pET16b-Trx CC32/35SS

Plasmid

#36946

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin 1 (TXN Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

both active site cysteines (32,35) mutated to ser…

Promoter

T7

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pEGB 35S:Luciferase:Tnos (GB0110)

Plasmid

#68216

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pB[U6-3 wex2 PUb-DsRed]

Plasmid

#169010

Purpose

For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

white ex2 sgRNA

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster U6:3

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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Sf-U7snRNA-ESE-AS-NsiI

Plasmid

#190694

Purpose

Basic vector used for the expression of U7-based antisense oligonucleotides

Depositor

Pim Pijnappel

Article

van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

Insert

U7 snRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U7 promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-CALPAIN-7-mCherry

Plasmid

#180652

Purpose

Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GCACCCAUACCUUUACAUU

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-29

Plasmid

#172753

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-29

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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