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Plasmid#165040PurposeExpression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)DepositorInsertEGFP
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-2(KW)2-eGFP
Plasmid#112736PurposeExpress 2(KW)2-eGFP in E. coliDepositorInsert2(KW)2-eGFP
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI
Plasmid#50723Purposeoverexpression of human TPI1 in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvCAHS12
Plasmid#192468PurposeBacterial expression of RvCAHS12DepositorInsertCAHS12
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11b-HA2
Plasmid#165992PurposeInfluenza HA ministem with removable HisTagDepositorInsertHA2
UseTagsFoldon and HisTagExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28GST-LIC
Plasmid#26101PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6XHis, GST, and Thrombin CLeavage SiteExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28b-Ava3855
Plasmid#45743DepositorInsertAva3855 (Ava_3855 Anabaena variabilis)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
NylCA-TS_pET28a
Plasmid#215419PurposeExpression construct for NylCA-TS gene, codon optimized for expression in E. coliDepositorInsertNylCA-TS
UseTagsExpressionBacterialMutationS111G/A137L/E263QPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2-TS_pET28a
Plasmid#215421PurposeExpression construct for NylCp2-TS gene, codon optimized for expression in E. coliDepositorInsertNylCp2-TS
UseTagsExpressionBacterialMutationD122G/H130Y/D36A/E263QPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
yPet_cp229-Binder
Plasmid#179137PurposeYPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).DepositorInsertSspB
UseTagsyPet_cp229ExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a(DAP10)
Plasmid#177904PurposeBacterial expression of his10-tagged human DAP10 Intracellular DomainDepositorInsertDAP10 (aa 70-92 only) (HCST Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluA-NP
Plasmid#165092Purposeprotein expression in E. coliDepositorInsertInfluenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET11b-MIF
Plasmid#149446PurposeExpression of WT MIF in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23a-HsUBB
Plasmid#69562PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertUBB (UBB Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDSacI
Plasmid#38255DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETM6-FAS
Plasmid#49810PurposeEncodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configurationDepositorInsertsfatty acyl-ACP thioesterase
hydroxydecanoyl-ACP dehydratase
b-ketoacyl-ACP synthase III
3-ketoacyl-ACP reductase
enoyl-(acyl carrier protein) reductase
UseTagsExpressionBacterialMutationCodon-optmized. See supplemental information of …PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtLeuRS
Plasmid#31079DepositorInsertMature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23 P53DBD
Plasmid#174503Purposeexpress P53 DNA binding domain (94-312) in E.coli without tagDepositorInsertP53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NM23-H1
Plasmid#157987PurposeExpresses NM23-H1 in E.coli cellDepositorInsertNM23-H1 (NME1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only