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pCI-neo-HA-hAtg14

Plasmid

#24294

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-hUVRAG

Plasmid

#24296

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV irradiation resistance-associated gene (UVRAG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV10-hUVRAG

Plasmid

#24292

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV irradiation resistance-associated gene (UVRAG Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pET21c(+)-mtPheRS

Plasmid

#31080

Depositor

Linda Spremulli

Article

Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

Insert

Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Nt …

Promoter

T7

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pET21c(+)-EFG1mt

Plasmid

#31076

Depositor

Linda Spremulli

Article

Bhargava et al Protein Expr Purif. 2004 Oct . 37(2):368

Insert

Mature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Includes the coding sequence for the mature form.…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-IP GFP-Atg14

Plasmid

#38264

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
pET28-MRPP1

Plasmid

#67862

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP1 (TRMT10C Human)

Use

Tags

His

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc human MOB1

Plasmid

#37024

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

MOB1B (MOB1B Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
CBP TAZ1(340-439)

Plasmid

#173760

Purpose

bacterial expression of mouse CBP TAZ1 domain

Depositor

Peter Wright

Article

De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

Insert

CBP TAZ1 (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-neo-Strep_Flag_Cterm

Plasmid

#102645

Purpose

Epitope tag c-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

Type

Empty backbone

Use

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-HA-hUVRAG

Plasmid

#24290

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV irradiation resistance-associated gene (UVRAG Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2010

Availability

Academic Institutions and Nonprofits only
pRcCMV Rootletin (Nigg pFL6(CW501))

Plasmid

#41167

Depositor

Erich Nigg

Article

Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

Insert

Rootletin (CROCC Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
Affibody EGFR:1907.BirA*-pET301/NT-DEST

Plasmid

#174691

Purpose

Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

Depositor

Jean Gariepy

Article

Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

Insert

Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*

Use

Tags

HIS tag

Expression

Bacterial

Mutation

R118G mutation in BirA

Promoter

T7 promoter

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro GFP-UVRAG

Plasmid

#38267

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV radiation resistance-associated gene protein (UVRAG Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pDONR221-OSF-TRIM5-21R

Plasmid

#79063

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5-21R

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

T7

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJOE5751.1

Plasmid

#135083

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-His tag-eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-CK2beta 6KR (pAB1016)

Plasmid

#37361

Depositor

David Litchfield

Article

French et al J Biol Chem. 2007 Oct 5;282(40):29667-77

Insert

CK2beta 6KR (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K33R, K139R, K177R, K191R, K208R, K212R

Promoter

CMV-F

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
CSII-IDR2-rhesusTRIM5-FOS

Plasmid

#79059

Purpose

Express rhesus TRIM5 in mammalian cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

rhesusTRIM5-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-Myc-hAtg14

Plasmid

#24293

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Tags

3xMyc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only