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Plasmid#123810PurposeEncodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertBgh pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(KD)
Plasmid#84640PurposemCherry-tagged, LKB1(K78I); kinase-dead mutantDepositorInsertmCherry-LBK1(K78I) (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationLysine 78 mutated to Isoleucine; kinase deadPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK3_026
Plasmid#123741PurposeEncodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK systemDepositorInsertFlamindo2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1899)
Plasmid#170143PurposeExpresses residues 1-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1900-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (787-1899)
Plasmid#170145PurposeExpresses residues 787-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-786; C-termin…PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (787-2138)
Plasmid#170146PurposeExpresses residues 787-2138 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-786; C-termin…PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1547)
Plasmid#170144PurposeExpresses residues 1-1547 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1548-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (936-2997)
Plasmid#170147PurposeExpresses residues 936-2997 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationN-terminal truncation of residues 1-935PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate-P2A-APEX2-NS
Plasmid#67670PurposeGateway entry clone containing mKate2-P2A-APEX2 with no stop codonDepositorInsertmKate2-P2A-APEX2-NS
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK0_030
Plasmid#123969PurposeEncodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK systemDepositorInsertphiC31-hygro-mRuby2PEST landing
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-KKHSaCas9-BlastR
Plasmid#107189PurposeConfers constitutive expression of KKH SaCas9.DepositorInsertKKH SaCas9 (NEWENTRY )
UseTagsExpressionBacterialMutationE782K/N968K/R1015HPromoterChicken ß-ActinAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK2_007
Plasmid#123702PurposeEncodes human pEF1alpha as a Type 2 part to be used in the MTK systemDepositorInsertpEF1a
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4_003
Plasmid#123812PurposeEncodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertrglb pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_008
Plasmid#123671PurposeEncodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-Orai1
Plasmid#89818PurposeExpress Orai1 in mammalian cellsDepositorInsertOrai1 (ORAI1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK5_012
Plasmid#123856PurposeEncodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK systemDepositorInsertConnector RE'-A1 Insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.0
Plasmid#139477PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTagsExpressionMammalianMutationV413LPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
JPF0510
Plasmid#124001PurposeEncodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_009
Plasmid#123672PurposeEncodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L1-2xHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK2_004
Plasmid#123699PurposeEncodes mouse pPGK1 as a Type 2 part to be used in the MTK systemDepositorInsertpPGK1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only