Addgene

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Showing: 1751 - 1800 of 16841 results

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pET-Gate2 mazEF

Plasmid

#117117

Purpose

Destination vector with mazEF toxin antitoxin system

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Type

Empty backbone

Use

Backbone for golden gate assembly for gene target…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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mVenus-N1

Plasmid

#54640

Purpose

Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mKikGR-C1

Plasmid

#54656

Purpose

Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Habuchi et al PLoS One. 2008;3(12):e3944. doi: 10.1371

Type

Empty backbone

Use

Tags

mKikGR

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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Active Wnt11-V5

Plasmid

#43824

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT11 (WNT11 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Venus-pBAD

Plasmid

#54859

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Use

Tags

6xHis and Venus

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Active Wnt7A-V5

Plasmid

#43816

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT7A (WNT7A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt1-V5

Plasmid

#43807

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

Wnt1 (WNT1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt4-V5

Plasmid

#43812

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT4 (WNT4 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS3867

Plasmid

#185744

Purpose

Sub-array backbone 3/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Active Wnt3-V5

Plasmid

#43811

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT3 (WNT3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pETconNK

Plasmid

#81169

Purpose

pETconNK Empty Backbone

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Type

Empty backbone

Use

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pWS3868

Plasmid

#185745

Purpose

Sub-array backbone 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Active Wnt2-V5

Plasmid

#43809

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT2 (WNT2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt9B-V5

Plasmid

#43821

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT9B (WNT9B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

M106T

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAN1201

Plasmid

#74695

Purpose

Circuit backbone plasmid (LacZa insert)

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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Active Wnt10B-V5

Plasmid

#43823

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT10B (WNT10B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
U6-TM2Emp-6T_iBlue

Plasmid

#200235

Purpose

Backbone for cloning short RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Active Wnt10A-V5

Plasmid

#43822

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT10A (WNT10A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
CIT32u

Plasmid

#128019

Purpose

K92A

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Use

Tags

Expression

Mutation

see Depositor Comments

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CIT0

Plasmid

#128017

Purpose

R66A

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Use

Tags

Expression

Mutation

see Depositor Comments

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSIIbsr-AKAR3EV

Plasmid

#170309

Purpose

The lentiviral vector for AKAR3EV

Depositor

Michiyuki Matsuda

Article

Komatsu et al Mol Biol Cell. 2011 Dec;22(23):4647-56.

Insert

AKAR3EV

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRA2

Plasmid

#49350

Purpose

Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU1

Plasmid

#72934

Purpose

Backbone vector with internal BsaI removed by site-directed mutagenesis

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBP

Plasmid

#72947

Purpose

Backbone vector with internal BsmBI removed by site-directed mutagenesis

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7RNAP-Q737P

Plasmid

#175356

Purpose

Support plasmid for T7_ori plasmids

Depositor

Sven Panke

Article

Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

Insert

T7RNAP-Q737P

Use

Tags

Expression

Bacterial

Mutation

Q737P

Promoter

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-mCA1-emptyMCS

Plasmid

#35095

Depositor

James Fleet

Article

Xue et al Mol Cancer Res. 2010 Aug;8(8):1095-104.

Type

Empty backbone

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

mouse carbonic anhydrase 1

Available since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pES E38A

Plasmid

#13049

Depositor

Peggy Hsieh

Article

Schofield et al J Biol Chem. 2001 Dec 7. 276(49):45505-8

Insert

MutS

Use

Tags

His6

Expression

Bacterial

Mutation

mutation Glu38 to Ala

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pICH82094

Plasmid

#48074

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEosEM-E_N1

Plasmid

#226337

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; empty backbone containing mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHPHS309

Plasmid

#228230

Purpose

GFP dropout backbone for sgRNA library cloning

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pgRNA_AT

Plasmid

#196243

Purpose

Backbone plasmid for cloning sgRNA

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41K

Plasmid

#194522

Purpose

Low copy vector backbone with G418 selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41N

Plasmid

#194524

Purpose

Low copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42N

Plasmid

#194525

Purpose

High copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41H

Plasmid

#194526

Purpose

Low copy vector backbone with hygromycin B selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42H

Plasmid

#194527

Purpose

High copy vector backbone with hygromycin B selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Bneu-9

Plasmid

#205214

Purpose

Unit Bactoneuron-9, expresses EGFP

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13014

Plasmid

#212685

Purpose

integration vector for single cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12712

Plasmid

#212683

Purpose

integration vector for single cassette with integration site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12711

Plasmid

#212682

Purpose

integration vector for single cassette with integration site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13015

Plasmid

#212686

Purpose

integration vector for single cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13016

Plasmid

#212687

Purpose

integration vector for single cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12713

Plasmid

#212684

Purpose

integration vector for single cassette with integration site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pML523

Plasmid

#203345

Purpose

Plasmid backbone for choline sensing circuits. The plasmid contains, lacI and tetR as well as betIM. The plasmid also contains the p15a ori

Depositor

Lauren Andrews

Article

Lebovich et al ACS Synth Biol. 2023 Aug 15. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pML3001

Plasmid

#203346

Purpose

Plasmid backbone for gamma-aminobutyric acid circuits. The plasmid contains gabR, lacI and tetR as well as the resistance gene kanR and the p15a ori.

Depositor

Lauren Andrews

Article

Lebovich et al ACS Synth Biol. 2023 Aug 15. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Landing_Pad_8xCTS-ECFP-pA

Plasmid

#200242

Purpose

Backbone for cloning 8xCTSs (8xCRISPR-target sites)

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CovKCR2_mCherry-pcDNA3.1

Plasmid

#201689

Purpose

expresses CovKCR2_mCherry fusion in mammalian cells

Depositor

John Spudich

Article

Govorunova et al mBio. 2022 Dec 20;13(6):e0303922. doi: 1

Insert

CovKCR2

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_72_TU6of6_X2homology

Plasmid

#194414

Purpose

TU6 backbone (for TU #6 for plasmid with 6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_77_TU1of2-6_HOhomology

Plasmid

#194416

Purpose

TU1 backbone (for TU #1 for plasmid with 2-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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