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Plasmid#55776PurposeThis G protein alpha-o construct contains internal insertions of YFP and the EE epitopeDepositorInsertG protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
UseTagsEE epitope was introduced internally with D167E a…ExpressionMammalianMutationA BglII site was removed from the 5' untrans…PromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA GST PreScission p70 S6K1
Plasmid#15511DepositorInsertp70 S6K1 (Rps6kb1 Rat)
UseTagsGST and HAExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
EGFPC2-CB2 SH3+
Plasmid#68821PurposeEGFP tagged Collybistin expression in mammalian cellsDepositorInsertCollybistin (Arhgef9 Rat)
UseTagsEGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-pHuji
Plasmid#107369PurposeSynaptic vesicle protein VAMP2 fused to the red FP pH sensor pHujiDepositorInsertVAMP2 (Vamp2 Rat)
UseTagspHujiExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-SnFR-gamma8-minWPRE
Plasmid#165498PurposeAAV for glutamate reporter fused to TARP gamma-8DepositorInsertiGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Plasmid#68717PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6s
UseAAVTagsExpressionMutationCre-dependent expression from inverted open readi…PromoterCAG-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-PSD95-N-14
Plasmid#57480PurposeLocalization: Membrane Scaffold, Excitation: 507 / 572, Emission: 516 / 580DepositorInsertPSD95
UseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Rlucrarr3-Flash1
Plasmid#74129PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eGFP-SynaptoZip
Plasmid#122525PurposeAAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoterDepositorInserteGFP-SynaptoZip (Vamp2 Rat)
UseAAVTagsMyc and eGFPExpressionMammalianMutationPromoterhSynAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTq2-Calmodulin
Plasmid#198200PurposeFRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2DepositorInsertCalmodulin 2 (Calm2 Rat)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Rims2-GFP KI
Plasmid#131495PurposeEndogenous tagging of RIM2: C-terminal (amino acid position: S1551)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Syt7 KI
Plasmid#131488PurposeEndogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ERK2 D319N
Plasmid#8979DepositorInsertERK2 (Mapk1 Rat)
UseTagsHAExpressionMammalianMutationD319NPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
PKC beta I WT
Plasmid#16378DepositorInsertPKC beta 1 (Prkcb Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-CAMKIIalpha-K42R
Plasmid#21221DepositorInsertCAMKIIalpha-K42R (Camk2a Rat)
UseTagsGFPExpressionMammalianMutationK42R (Lab plasmid KS 0007).PromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash4
Plasmid#74132PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
RIP-Timer (DM#285)
Plasmid#15109DepositorInsertinsulin II promoter (Ins2 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only