-
Plasmid#23802DepositorInsertSTK11 (STK11 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
RSRF-Luc-2wt
Plasmid#31818DepositorInsertMEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseTagsExpressionMammalianMutationPromoterMEF2-dependent (-307 to -242 of Nur77/NR4A1)Available sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcsII-LMP7-flag
Plasmid#86773PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NfiA
Plasmid#64901PurposeInducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.DepositorInsertNfia (Nfia Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2attNG-H11-long
Plasmid#51545Purposep2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.DepositorInsertGFP4m
UseHuman targetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7_hsa-miR-34a
Plasmid#25791DepositorInserthsa-miR-34a (TP53 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBA409
Plasmid#85971PurposeEF1a-sfGFP-Ubc-NeoDepositorInsertssfGFP
Neo
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7b1(glu)
Plasmid#14673DepositorInsertMKK7b1(Glu) (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationSer-269Glu Thr-273GluPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP
Plasmid#11153PurposeMammalian expression vector for expression of GFP (CMV promoter)DepositorHas ServiceCloning Grade DNAInsertCMV promoter-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-P2A-tdTomato
Plasmid#72486PurposeExpress Luc2 and tdTomato under EF1 promoterDepositorInsertLuc2-P2A-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA
Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TCF1
Plasmid#40620DepositorInsertTCF7 (TCF7 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLBH531_MBP-Cas14a1 expression
Plasmid#112500PurposeExpresses MBP fused to Cas14a1DepositorInsertCas14a1
UseCRISPRTags10xHis-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFlagCMV2-EFP
Plasmid#12449DepositorInsertEFP (TRIM25 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV FKBP-V5-sTurboID (N)
Plasmid#153002PurposeExpresses low-affinity split-TurboID N-terminal fragment in mammalian cellsDepositorInsertsTurboID (N) (L73)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV HA-HaloTag-FRB-sTurboID (C)
Plasmid#153003PurposeExpresses low-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID (C) (G74)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationKO: all lysines mutated to argininesPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-YAP1
Plasmid#66853PurposeExpress FLAG-tagged YAP1 in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Hygro
Plasmid#127763PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only