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Plasmid#139865PurposeLentiviral expression of HA-tagged human SEC62DepositorInsertSEC62 (SEC62 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD1
Plasmid#20183DepositorInsertNotch 1 Intracellular Domain (Notch1 Mouse)
UseTags3XFLAGExpressionMammalianMutationContains murine Notch 1 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
LD101: pMVP (L3-L2) eCFP + WPRE
Plasmid#121775PurposepMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.DepositorInserteCFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-TEX264-HA
Plasmid#139866PurposeLentiviral expression of HA tagged (C terminal) human TEX264DepositorInsertTEX264 (TEX264 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
TPST1-EGFP
Plasmid#66617PurposeEncodes GFP fusion of TPST1 trans Golgi marker; (Tyrosyl protein sulfotransferase 2)DepositorInsertTPST1 (TPST1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB554
Plasmid#74884PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVDL9.3
Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
UseTagsExpressionBacterialMutationF243I/D238C/S283C/Y127GPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS:mCherry
Plasmid#170994PurposeUAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.DepositorInsertUAS:mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterSynthetic 14x UAS:Gal4Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCB306-GFP-Rab29
Plasmid#198472Purposelentiviral expression of GFP-Rab29DepositorInsertGFP-Rab29 (RAB29 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetOn-REEP5-mCherry-eGFP
Plasmid#139870PurposeDox-inducible lentiviral expression of human REEP5DepositorInsertREEP5 (REEP5 Human)
UseLentiviralTagsmCherry-eGFPExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROP-sgRNA-MS2
Plasmid#153457PurposeCROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffoldDepositorInsertsgRNA empty backbone
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDualEX-SMN2
Plasmid#214431PurposeDual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7DepositorInsertSMN2 EXON 7 (SMN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD2
Plasmid#20184DepositorInsertNotch 2 intracellular domain (Notch2 Mouse)
UseTags3X FLAGExpressionMammalianMutationContains murine Notch 2 Intracellular Domain (Met…PromoterCMVAvailable sinceJan. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGWB533
Plasmid#74872PurposeGateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB402
Plasmid#74796PurposeGateway cloning compatible binary vector for expression of gene by CaMV35S promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB502
Plasmid#74844PurposeGateway cloning compatible binary vector for expression of gene by CaMV35S promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ST-SBP-EGFP
Plasmid#65264Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
human-GFAP-EGFP
Plasmid#169473PurposeWe generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.DepositorInsertEGFP-hGFAP (GFAP Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-GFP
Plasmid#106281PurposeA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.DepositorInsertCMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only