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Plasmid#74808PurposeGateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only
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mGarnet2-alpha-tubulin
Plasmid#104318Purposecodes for a fusion of fluorescent protein mGarnet2 and alpha-tubulin, visualizes alpha-tubulinDepositorInsertmGarnet2-alpha-tubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSV2neo human IFN-gamma
Plasmid#10954DepositorInsertinterferon-gamma (IFNG Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-modified
Plasmid#84477Purposeguide RNA expression lentiviral constructDepositorInsertguide RNA
UseLentiviralTagsExpressionMammalianMutationPromotermU6Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
m6A-Tracer-NES
Plasmid#159607PurposeConstitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.DepositorInsertm6A-Tracer-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mNeonGreen
Plasmid#137802PurposeVisualization of microtubulesDepositorArticleInsertEnsconsin microtubule binding domain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
opto-Tiam1
Plasmid#174508PurposeExpresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.DepositorInsertopto-Tiam1-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSVBneoT
Plasmid#22883DepositorInsertEarly region SV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
PIP2-GFP(ST) sensor
Plasmid#170356PurposeGFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.DepositorInsertGFP(ST)-PH (PLCD1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pRSET-b
Plasmid#188446PurposeB-gTEMP for bacterial expressionDepositorInsertB-gTEMP
UseTags6xHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-RPS27A-UTR-PP7-WPRE
Plasmid#198339PurposePB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loopsDepositorInsertRPS27A-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loopsExpressionMammalianMutationPromoterEf1aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
UseTagsHA tag and myc tagExpressionYeastMutationPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2948
Plasmid#144424PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXB6 (HOXB6 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
Plasmid#214102PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AbVec_IGHC
Plasmid#183701PurposeEncode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.DepositorInsertIGHG1 (IGHG1 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GZnP3 in pcDNA3.1+
Plasmid#161738PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)DepositorInsertGZnP3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_WT
Plasmid#98653PurposeExpresses MBP-tagged FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.4_17G6-1 heavy chain
Plasmid#198249Purposerecombinant expression of heavy chain of monoclonal anti-AMP antibody 17G6-1DepositorInsertheavy chain of monoclonal anti-AMP antibody 17G6-1 (mouse IgG2b)
UseTagsIL10 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAB10 T23N
Plasmid#130885PurposeExpresses human RAB10 T23N mutant with N-terminal GFP tagDepositorInsertRAB10 (RAB10 Human)
UseLentiviralTagsGFPExpressionMammalianMutationT23NPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 FLAG-NGRN-Fc
Plasmid#115773PurposeExpression of the extracellular domain of NGRN with N-terminal FLAG tag and C-terminal hIgG tag.DepositorInsertNGRN (NGRN Human)
UseTagsFLAG and hIgG/FcExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-cyto-GCaMP8f
Plasmid#186050PurposeAAV-mediated expression of GCaMP8f under the control of GfaABC1D promoterDepositorInsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterGfaABC1DAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
LEPG
Plasmid#111160PurposemiR-E (miR-30 variant)-based RNAi with GFP reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-IRESWT-Gbetagamma ONE-GO
Plasmid#204339PurposeGPCR/G protein BRET biosensorDepositorInsertGbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657
Plasmid#212962PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_non-targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0051-W85X REPAIR targeting guide cloned into pC0043
Plasmid#103867PurposeGuide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038DepositorInsertW85X targeting guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC34A2
Plasmid#132088PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC34A2 (SLC34A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLeAPS-GFP-blast
Plasmid#182230PurposeLentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgeneDepositorInsertGFP-P2A-Blasticidin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only