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Showing: 7161 - 7180 of 148587 results

  2. IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

    Plasmid
    #121750
    Purpose
    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    3x HA epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121750-plasmid-map-sequence-id-237188

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  7. plRL61

    Plasmid
    #163633
    Purpose
    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tet-On
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163633-plasmid-map-sequence-id-339483

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  9. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  11. pGD-γb

    Plasmid
    #196327
    Purpose
    35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    barley stripe mosaic virus γb
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196327-plasmid-map-sequence-id-390033

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  15. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

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  19. pYTK077

    Plasmid
    #65184
    Purpose
    Encodes KanamycinR as a Type 6 part to be used in the Dueber YTK system
    Depositor
    John Dueber
    Article
    Lee et al ACS Synth Biol. 2015 Apr 14.
    Insert
    KanamycinR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65184-plasmid-map-sequence-id-110237

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Showing: 7161 - 7180 of 148587 results