We narrowed to 9,435 results for: try

1. FAK2_HUMAN_D0

   Plasmid

   #79747

   Purpose

   This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   FAK2 (PTK2B Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pPB-hCMV1-hKLF2-IV

   Plasmid

   #60433

   Purpose

   tet-inducible human KLF4

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   KLF2 (KLF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ265E6

   Plasmid

   #213469

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cda-32aa linker-z…

   Tags

   No

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

   Plasmid

   #104097

   Purpose

   Expresses TREAT-PK co-localization control in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDule2-3-nitroTyrosine (5B)

   Plasmid

   #85499

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b-Caspase-3(D3A: D175A D28A D9A)

   Plasmid

   #90090

   Purpose

   Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

   Depositor

   Clay Clark

   Article

   Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D175A D28A D9A

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. JE301: pMVP (L5-L4) Tir1-P2A-AID

   Plasmid

   #121722

   Purpose

   pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   osTir1-P2A-AID (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   9x myc (C-term on osTIR1)

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR1A mKate2-hu_ncOGT K842M

   Plasmid

   #154284

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   K842M mutation (Catalytic Inactive)

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBacI-KDAC8 D101E

   Plasmid

   #224350

   Purpose

   Expresses human KDAC8 (HDAC8) D101E in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

   Insert

   KDAC8 (HDAC8 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Aspartate 101 mutated to glutamate

   Promoter

   Polyhedrin

   Available since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pENTR4-EGFP_opt

    Plasmid

    #106334

    Purpose

    Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    codon optimized for gene synthesis without affect…

    Promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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