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Plasmid#115875PurposeMPHOSPH8 knockdownDepositorInsertMPHOSPH8 shRNA (MPHOSPH8 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
[UBC][firefly luciferase][target cloning][renilla luciferase]
Plasmid#97190PurposeReporter for translation activation via Pum.DepositorInsertsFirefly Luciferase
Renilla Luciferase
UseSynthetic BiologyTagsnoneExpressionMammalianMutationPromoterUBC (ubiquitin C)Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT for Split-PE (pEH45)
Plasmid#190105PurposeExpresses MMLV-RT (full-length, from PE2) for Split-PE useDepositorInsertMMLV-RT
UseTagsbpNLSExpressionMammalianMutationRT pentamutant from PE2 (D200N, T306K, W313F, T33…PromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
neurod1:Gal4; cmlc2:EGFP
Plasmid#173478PurposeGal4 is driven by -5kb neurod1 promoter. The vector contains a cmlc2:EGFP markerDepositorInsertGal4
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-MPHOSPH8 ts3
Plasmid#115876PurposeMPHOSPH8 knockdownDepositorInsertMPHOSPH8 shRNA (MPHOSPH8 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 (RNAi-res)
Plasmid#136645PurposeExpression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1 (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTCPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-HNHx-ABE8eHD-BlastR
Plasmid#176478PurposeA-to-G base editor with alternative targeting scopeDepositorInsertHNHx-ABE8eHD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BICD1
Plasmid#49487Purposeexpresses EGFP-BICD1 in mammalian cellsDepositorInsertBICD1 (BICD1 Human)
UseTagsEGFPExpressionMammalianMutation** see notesPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-YAP1
Plasmid#79503PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDSDepositorInsertYAP1 (YAP1 Human)
UseMultisite gateway entry cloneTagsFLAGExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAIP Nup62Fv2GFP
Plasmid#139684PurposeNup62 dimerization construct fused to 2 copies of GFP under an SFFV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIC292
Plasmid#51405PurposeRetroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)DepositorInsertSka2 IMAGE 4685972 (SKA2 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)
Plasmid#136646PurposeExpression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1R312A (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MTS-WT-c-Src-FLAG
Plasmid#44652DepositorInsertv-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
UseTagsFLAG tag and mitochondria-targeting sequence (res…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr230-EGFP
Plasmid#58472PurposeExpresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoterDepositorInsertUtr230 (UTRN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
P445-FBP_17
Plasmid#139708PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlledDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
RIPR-bio-His
Plasmid#50824PurposeExpresses enzymatically monobiotinylated full length RIPRDepositorInsertRIPR
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
RS18960_pMQ30_KO_Kan
Plasmid#185393PurposeNon-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11DepositorInsertsRR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only