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Plasmid#104824PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.08g081600, Glyma.05g126600
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hU6-cs1-PuroR-T2A-BFP
Plasmid#230934PurposegRNA cloning vector with direct capture sequence 1 in stem loop 2DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsTagBFPExpressionMutationPromoterAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDL655
Plasmid#231165PurposeT-DNA encoding gRNA targeting SlPDSDepositorInsertgRNA targeting SlPDS
UseTagsExpressionPlantMutationPromoterAtU6Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-C9orf72
Plasmid#83439PurposeExpresses Cas9 and a gRNA targeting C9orf72DepositorInsertgRNA targeting human C9orf72 (C9orf72 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Hbb
Plasmid#99693PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B-Pho2-1/2-2-tRNA
Plasmid#161761PurposeMODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)DepositorInsertgRNAs
UseTagsExpressionBacterialMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067L
Plasmid#200253PurposepBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomatoDepositorInsert6 gRNAs targeting Doublesex, Intersex, bTublin
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-sg-1
Plasmid#190607PurposeExpresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherryDepositorInsertgRNA sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW037
Plasmid#185688PurposeT-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAsDepositorInsertCas9, Drm1b gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker-3xFLAG KI
Plasmid#182679PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.DepositorInsertNefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC45
Plasmid#104819PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC29
Plasmid#104803PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr4g094545
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC39
Plasmid#104813PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101180 (Cop-like). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr2g101180
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC34
Plasmid#104808PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr8g060730 (Fbl1-like). Also expresses Cas9 from AtUBQ10 promoterDepositorInsertMedtr8g060730
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC33
Plasmid#104807PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr8g060730 (Fbl1-like). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr8g060730
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC25
Plasmid#104799PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from Gmubi promoter from Gmubi promoterDepositorInsertMedtr2g101040
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC24
Plasmid#104798PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoterDepositorInsertMedtr2g101120, Medtr2g101130
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC22
Plasmid#104796PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoterDepositorInsertMedtr2g101120, Medtr2g101130
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC19
Plasmid#104793PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from Gmubi promoter from Gmubi promoterDepositorInsertMedtr2g020630
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP
Plasmid#91879PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2DepositorInsertgRNA targeting Ezh2 (Ezh2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only