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Plasmid#157686Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp5 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-GP-2-KDM4A double Tudor
Plasmid#59699PurposeContains histone modification interacting domain (KDM4A double Tudor)DepositorInsertKDM4A double Tudor (KDM4A Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
ENDOD1-CD4d3+4-bio
Plasmid#73114PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding ENDOD1 with rat CD4d3+4-bioDepositorInsertENDOD1 (ENDOD1 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a_HOXA10_P2A_Hygro_Barcode
Plasmid#120452PurposeBarcoded lentiviral vector to express HOXA10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertHOXA10 (HOXA10 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD55-CD4d3+4-bio
Plasmid#73116PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CD55 with rat CD4d3+4-bioDepositorInsertCD55 (CD55 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet-H_N1
Plasmid#85058PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmScarlet-HExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Orf6
Plasmid#157727Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoterDepositorInsertFH-Orf6 (ORF6 SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSc-His6-Sem1Sc
Plasmid#83235PurposepGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeastDepositorInsertSem 1 (SEM1 Budding Yeast)
UseTagsHis6ExpressionYeastMutationPromoterAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F2shRNA human
Plasmid#66884PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F2DepositorInsertE2F transcription factor 2 (E2F2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS3-MT-BX/p14ARF
Plasmid#78761PurposeTo overexpress p14ARF in Mammalian CellsDepositorInsertARF (CDKN2A Human)
UseTags6xMYCExpressionMammalianMutationPromoterCMV IE94Available sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Hygro-sgSIK3
Plasmid#138699PurposeExpresses a human SIK3-targeting sgRNA and Cas9DepositorInsertsgSIK3 human (SIK3 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO HA-AMPKa2 AS
Plasmid#69827PurposeExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible mannerDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterminimal CMV promoter with tetracycline operatorAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-OB-Linker-eGFP
Plasmid#176068PurposeEGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassetteDepositorInsertOB
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)
Plasmid#140535PurposeOsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-FLAG-TAOK2
Plasmid#197862PurposeExpression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cellsDepositorInsertTAOK2 (TAOK2 Human)
UseTagsGFPExpressionMammalianMutationWild TypePromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-SIK2
Plasmid#138696PurposeExpresses a human SIK2-targeting sgRNA and Cas9DepositorInsertsgSIK2 human (SIK2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-SIK3
Plasmid#138697PurposeExpresses a human SIK3-targeting sgRNA and Cas9DepositorInsertsgSIK3 human (SIK3 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-eDHFR-Cdc42Q61L
Plasmid#107267PurposemCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.DepositorUseTagsmCherryExpressionMammalianMutationPromoterT7Available sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AchE-CD4d3+4-bio
Plasmid#73119PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding AchE with rat CD4d3+4-bioDepositorInsertAchE (ACHE Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only