Addgene

Showing: 7141 - 7160 of 11900 results

Results per page:

Sort results by:

p198 Dync1i2.F (Ex1a)

Plasmid

#26448

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

N/A

Promoter

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-G1238A-C1240T

Plasmid

#24739

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

G replaced with A at position 1238 and C replaced…

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
HsCLYBL(aa23-340)-His

Plasmid

#111288

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL ( aa23-340) codon optimized for bacterial expression (CLYBL Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK048

Plasmid

#75230

Purpose

Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Use

Tags

Expression

Bacterial

Mutation

Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

Promoter

T7 and n/a

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
HM3C NaVSp1 pET24b

Plasmid

#128088

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

Depositor

Dan Minor

Article

Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

Insert

NaVSp1p

Use

Tags

3C Cleavage, His Tag, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pSI-Check2-hRluc-NFkB-firefly

Plasmid

#106979

Purpose

Luciferase reporter with internal control for NFkB signal.

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

NFKB recognition sites (NFKB1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIC28-DENV3-NS5FL

Plasmid

#86013

Purpose

Expression of dengue virus 3 NS5 protein

Depositor

Dahai Luo

Article

Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

Insert

DENV3-NS5FL

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

amino acid residues 6 to 895 of DENV3 NS5

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
DPYSL2-pLJC2

Plasmid

#189866

Purpose

Expresses human DPYSL2 (CRMP2) in mammalian cells

Depositor

Yoav Shaul

Article

Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

Insert

DPYSL2 (DPYSL2 Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EXT1-pLJC2-3XFLAG

Plasmid

#215818

Purpose

Ectopic expression of EXT-1 in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
p7SL-neo-TET

Plasmid

#51285

Purpose

expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

7SL RNA (RN7SL2 Human)

Use

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Mutation

Promoter

7SL upstream pollII enhancer

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-OSF-KATNA1

Plasmid

#160051

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
T7opt in pCAGGS

Plasmid

#65974

Purpose

Human codon-optimized T7 polymerase

Depositor

Benhur Lee

Article

Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

Insert

T7-opt

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-m

Use

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe hygro 3xFLAG Dna2 wt

Plasmid

#31955

Depositor

Sheila Stewart

Article

Duxin et al J Biol Chem. 2012 Jun 22;287(26):21980-9

Insert

hDna2 (DNA2 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Changed start ATG to AAA,

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p11d-tRPA-32Asp8

Plasmid

#102617

Purpose

Expression of human RPA with aspartic acid substituted phosphorylation domain

Depositor

Marc Wold

Article

Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

Inserts

RPA1 (RPA1 Human)
RPA3 (RPA3 )
RPA2 (RPA2 )

Use

Tags

Expression

Bacterial

Mutation

with aspartic acid substituted phosphorylation do…

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-REGv1(mCherry-FLAG) in pTwist-CMV

Plasmid

#216165

Purpose

Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression.

Depositor

Pietro Fratta

Article

Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.

Insert

mCherry with C-terminal FLAG tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe EGFR(L858R/T790M)

Plasmid

#32073

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L858R/T790M

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge