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pDONR223 SARS-CoV2 TEV-N

Plasmid

#154424

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

N (N SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MERTK_HUMAN_D0

Plasmid

#79705

Purpose

This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MERTK (MERTK Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221 SARS-CoV-2 S-frag5_nostop

Plasmid

#153177

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Mikko Taipale, Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag5

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-ORF3B

Plasmid

#154417

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector, with insert of o…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 7, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

Plasmid

#114088

Purpose

U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for MbCas12a crRNAs

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 SARS-CoV-2 S-frag3_nostop

Plasmid

#153175

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Mikko Taipale, Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag3

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221 SARS-CoV-2 S-frag4_nostop

Plasmid

#153176

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Mikko Taipale, Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag4

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221 SARS-CoV-2 S-frag1_nostop

Plasmid

#153173

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Mikko Taipale, Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag1

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pKK-TEV-mTagBFP

Plasmid

#105789

Purpose

Expression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mTagBFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-BI16-TEV-mCherry

Plasmid

#105800

Purpose

Concomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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