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Showing: 7121 - 7140 of 7946 results

  1. B-HeFSpCas9-A1003K

    Plasmid
    #207371
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1003K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …
    Promoter
    CBh
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207371-plasmid-map-sequence-id-408916

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  2. B-HeFSpCas9-A848K

    Plasmid
    #207369
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A848K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207369-plasmid-map-sequence-id-408749

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  3. B-HeFSpCas9-A661R

    Plasmid
    #207367
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207367-plasmid-map-sequence-id-408748

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  4. B-evoSpCas9-Q661R

    Plasmid
    #207366
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-Q661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207366-plasmid-map-sequence-id-408747

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  5. B-evoSpCas9-N515Y

    Plasmid
    #207364
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-N515Y
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, K526E, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207364-plasmid-map-sequence-id-408746

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  19. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

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Showing: 7121 - 7140 of 7946 results