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Plasmid#69821PurposeExpresses SNAP29-V5 in mammalian cellsDepositorInsertSynaptosomal-associated protein 29 (SNAP29 Human)
UseRetroviralTagsV5ExpressionMammalianMutationPromotercmvAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-T38M-750-luxAB-DAS+4
Plasmid#49520PurposeHygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomallyDepositorInsertT38M-P750-luxAB-DAS+4
UseTagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSV-CaMKIV(1-313)
Plasmid#45063PurposeExpression of constit active CaMKIVDepositorInsertCam Kinase IV(1-313) (Camk4 Mouse)
UseTagsExpressionMammalianMutationtruncated at amino acid 313*PromoterRSVAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF10_P2A_Hygro_Barcode
Plasmid#120496PurposeBarcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF10 (KLF10 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-Phobos
Plasmid#98166Purposeblue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos gene
UseTagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA
Plasmid#156441PurposeFor transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2DepositorInsertmouse SA2 (Stag2 Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp12-HF
Plasmid#157711Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoterDepositorInsertNsp12-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant
Plasmid#149507PurposeTransient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.DepositorInsertMFSD12 (MFSD12 Human)
UseTransient expressionTagsTEV-3XHA-3XFLAGExpressionMammalianMutationCodon Optimized Sequence, p.LL256-257AAPromoterCMVAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK341
Plasmid#192640PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyTagsExpressionMutationSoxS has R93A and S101A mutations and dSpRY has s…PromoterBBa_J23107 and Sp.pCas9Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-flex-Clover
Plasmid#135177PurposeAAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent lablingDepositorInsertsClover
TRE3G
UseAAVTagsExpressionMammalianMutationa variant of GFPPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-INPP4B C842A mutant
Plasmid#161996PurposeMammalian expression vector containing a phosphatase dead mutant of INPP4B (C842A). Also contains mCherry tagDepositorInsertINPP4B (INPP4B Human)
UseTagsmCherryExpressionMammalianMutationC842APromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert
Plasmid#124660Purposereporter protein which emits bright blue fluorescenceDepositorInsertGaussia luciferase-C9D-TGinsert
UseTagsIEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…ExpressionBacterialMutationchanged glutamic acid 100 to alanine, and glycine…PromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCcnb1
Plasmid#162793PurposeCcnb1 shRNA in pMKO.1 retroviral vectorDepositorInsertCcnb1 shRNA (Ccnb1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-SIK1
Plasmid#138695PurposeExpresses a human SIK1-targeting sgRNA and Cas9DepositorInsertsgSIK1 human (SIK1 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgSIK2
Plasmid#138708PurposeExpresses a human SIK2-targeting sgRNA and Cas9DepositorInsertsgSIK2 human (SIK2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)
Plasmid#140533PurposeOsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRQR-HF1 variant
Plasmid#138563PurposeExpresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSDDepositorInsertVRQR-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag
Plasmid#199457PurposeExpression of dCas13-VPR in mammalian cellsDepositorInsertdCas13
UseLentiviralTagsVPR-T2A-HaloTagExpressionMammalianMutationNuclease-dead H133A and H1058APromoterCAGAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
ICAM4-CD4d3+4-bio
Plasmid#73102PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding ICAM4 with rat CD4d3+4-bioDepositorInsertICAM4 (ICAM4 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt
Plasmid#157691Purposemammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits