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Plasmid#33089DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS163A, S164APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHtrA-TEV-His12
Plasmid#137036Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66500.2 (BB_0104 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp5-HF
Plasmid#157687Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoterDepositorInsertNsp5-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp2
Plasmid#157683Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp2 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hy_pMT Laccase2 Exons 1-3
Plasmid#91799PurposeDrosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNADepositorInsertLaccase2 (stw Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-ATRX ADD
Plasmid#59698PurposeContains histone modification interacting domain (ATRX ADD)DepositorInsertATRX ADD (ATRX Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp16-HF
Plasmid#157725Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoterDepositorInsertNsp16-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1-shGBP1.1.mKO2
Plasmid#85209PurposeTRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.DepositorInsertGBP1 (guanylate binding protein 1) (GBP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGMC00034 (aka pMC0235)
Plasmid#210751PurposeNon-targeting guide RNADepositorInsertNon-targeting guide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lactotransferrin-CD4d3+4-bio
Plasmid#73124PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Lactotransferrin with rat CD4d3+4-bioDepositorInsertLactotransferrin (LTF Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJEP303-pAAV-CMV-dSaCas9-VP64-pA
Plasmid#113678PurposeA CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted regionDepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Synthetic BiologyTagsNLS and VP64ExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet-i_N1
Plasmid#85059PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmScarlet-iExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
ALARM NMR
Plasmid#90209PurposeExpresses the human La antigen for use in ALARM NMRDepositorInsertLa antigen (SSB Human)
UseTags6xHisExpressionBacterialMutationcontains amino acids 100-324PromoterT7 promoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (F118D) FLAG GFP
Plasmid#164979PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorInsertFLCN (FLCN Human)
UseLentiviralTags1xFLAG-GFPExpressionMammalianMutationF118DPromoterUBCAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECE HA AMPKa1 AS
Plasmid#69831PurposeExpresses Expresses AMPK alpha 1 in mammalian cellsDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseTagsHAExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF6_P2A_Hygro_Barcode
Plasmid#120492PurposeBarcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF6 (KLF6 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Tfap2c
Plasmid#128826Purposedox-dependent expression of mouse Tfap2cDepositorInsertTfap2c (Tfap2c Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-myc-LC3-HA
Plasmid#45244DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsHA and mycExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0323-ss
Plasmid#137067PurposeE. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66700.1
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0323: lacks the BB0323 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13
Plasmid#157713Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp13 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits