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Plasmid#66883PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F1DepositorInsertE2F transcription factor 1 (E2F1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSCT (JBEI-11557)
Plasmid#87937PurposeCo-expression in S.cerevisiae of 4CL5 and AtSCTDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Chicken Mermaid S188
Plasmid#53617PurposeGenetically encoded voltage sensor based on mUKG-mKOk FRET pairDepositorInsertChicken Mermaid S188
UseTagsExpressionMammalianMutationGg-VSP contains an R153Q mutation and an amino ac…PromoterCMVAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
UCP3 promoter EP1
Plasmid#71743PurposeContains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activityDepositorInsertUncoupling Protein 3 promoter (Ucp3 Mouse)
UseTagsLuciferaseExpressionBacterialMutationContains 7kb of the murine UCP3 gene encompassing…PromoterUCP3Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13_GC3opt
Plasmid#157715Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS5-3HA/p14ARF
Plasmid#78764PurposeTo overexpress p14ARF in Mammalian CellsDepositorInsertp14ARF (CDKN2A Human)
UseTags3xHAExpressionMammalianMutationPromoterCMV IE94Available sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp10-HF
Plasmid#157708Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoterDepositorInsertNsp10-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
bio-CD4d3+4-TMEM24
Plasmid#73092PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding TMEM24 with bio-rat CD4d3+4DepositorInsertTMEM24 (C2CD2L Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHSN501
Plasmid#50589PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertszCas9D10A
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSExpressionMutationCas9D10A nickase, was derived from the zCas9 and …Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
GIV-FLAG S245A (SA)
Plasmid#86095PurposeExpression GIV in mammalian cellDepositorInsertGIV/Girdin (CCDC88A Human)
UseTags3xFlagExpressionMammalianMutationS245APromoterCMVAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human b4 nicotinic receptor subunit EM construct
Plasmid#163961PurposepEZT-BM vector with beta4 gene after CMV promoter, published EM constructDepositorInsertHuman beta4 nicotinic acetylcholine receptor subunit EM construct
UseBaculovirus (bacmam) production and mammalian exp…TagsStrepII tag and apocytochrome b(562)RIL (BRIL)ExpressionMammalianMutationPro341-Ser395 replaced with apocytochrome b(562)R…PromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp12
Plasmid#157710Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp12 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRD405
Plasmid#163110PurposeExpression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mScarlet-I_H-NSdbd_NLS
UseEukaryotic expressionTagsExpressionMammalianMutationPromoterCMV promoter; T7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgNUAK1
Plasmid#138692PurposeExpresses a human NUAK1-targeting sgRNA and Cas9DepositorInsertsgNUAK1 human (NUAK1 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_HAND2_P2A_Hygro_Barcode
Plasmid#120447PurposeBarcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertHAND2 (HAND2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiCas9-msa
Plasmid#164833PurposemiCas9-msa fusion protein expressing plasmidDepositorInsertmiCas9-msa
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationcontains mSA sequence range is 5106-5447 and Brex…PromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (R164A) FLAG GFP
Plasmid#164980PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorInsertFLCN (FLCN Human)
UseLentiviralTags1xFLAG-GFPExpressionMammalianMutationR164APromoterUBCAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD421
Plasmid#163112PurposeProduction of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysSDepositorInsertHis-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
UseTagsHis-tag with TEV-cleavable linkerExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Semaphorin7A-CD4d3+4-bio
Plasmid#73115PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bioDepositorInsertSemaphorin 7A (SEMA7A Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
GlycophorinA-CD4d3+4-bio
Plasmid#73093PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinA with rat CD4d3+4-bioDepositorInsertGlycophorin A (GYPA Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only