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pmCherry-Synaptojanin-1-145
Plasmid#223563PurposeExpresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminusDepositorInsertSYNJ1 (SYNJ1 Human)
UseTagsHis and mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-eSpCas9(1.1)
Plasmid#178800PurposeHigh-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-SpCas9-HF1
Plasmid#178801PurposeHigh-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR
Plasmid#221447PurposeLentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
UseLentiviralTagseGFPExpressionMammalianMutationSPR degron: hGEM (1-110) fragment that is fused t…PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
plenti hSyn AldoC
Plasmid#220917PurposeNeuronal specific expression of AldoCDepositorInsertAldoC (ALDOC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn HK1
Plasmid#220915PurposeNeuronal specific expression of HK1DepositorInsertHK1 (HK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-NES-jRGECO1a-WPRE
Plasmid#216276PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1aDepositorInsertNES-jRGECO1a
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-2
Plasmid#210136Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-1
Plasmid#210135Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 pCAGGS
Plasmid#206101Purposeexpression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expressionDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW449
Plasmid#198805PurposeDirect light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.DepositorInsert15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW539
Plasmid#198811PurposeDominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.DepositorInsert15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationG310D mutation to abolish the binding of cAMP of …PromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)
Plasmid#195552PurposeExpresses Thbs1 under control of short GFAP promoterDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromotershort GFAPAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iEscSnFR_PM
Plasmid#182813PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iEscSnFR_cyto
Plasmid#182814PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_cyto
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iFluoxSnFR_ER
Plasmid#182815PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iFluoxSnFR_PM
Plasmid#182816PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iFluoxSnFR_cyto
Plasmid#182817PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_cyto
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iEscSnFR_ER
Plasmid#182818PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_ER
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only
