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Plasmid#61534PurposeExpresses Hes1 in mammalian cellsDepositorInsertHes1 (Hes1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
bio-CD4d3+4-APMAP
Plasmid#73091PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding APMAP with bio-rat CD4d3+4DepositorInsertAPMAP (APMAP Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104096PurposeExpresses TREAT-ARE reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…ExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-fDIO-GRAB_eCB2.0
Plasmid#164610PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cellsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterEFSAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SMC2-mAC donor (Hygro)
Plasmid#140648PurposeSMC2 tagging with mAID-CloverDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
EphA2-MT
Plasmid#108851PurposeEncodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linkerDepositorInsertEPHA2 (EPHA2 Human)
UseTagsLabeled with mTurquoise on the C-Terminus via a 1…ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRAF_S259A_pcDNA3.1
Plasmid#86505PurposeExpresses CRAF_S259A in mammalian cellsDepositorInsertCRAF (RAF1 Human)
UseTagsFLAGExpressionMammalianMutationS259APromoterCMVAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:BRAF
Plasmid#118846PurposeExpresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertBRAF V600E (BRAF Zebrafish, Human)
UseZebrafish expression, tol2TagsExpressionMutationBRAF V600EPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA
Plasmid#192160PurposeDual AAV vector AAV-dCas9DepositorInsertDead Cas9
UseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartatePromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(D256A)-IRES-puro
Plasmid#128677Purposemammalian expression of Flag-Polß(D256A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationD256APromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-GFP-LoxP-SNAP-TERT
Plasmid#71391PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.DepositorInsertTERT (TERT Human)
UseHr donorTagsFLAG-SNAPExpressionMutationPromoterEndogenous TERT promotorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAC-TMPRSS4
Plasmid#172422PurposeMAC-tagged gene expressionDepositorInsertTransmembrane protease serine 4 (TMPRSS4 Human)
UseTagsMAC-tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICSL11060
Plasmid#68264PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)DepositorInsertPromoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_FOXP1_P2A_Hygro_Barcode
Plasmid#120441PurposeBarcoded lentiviral vector to express FOXP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertFOXP1 (FOXP1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-GsCT
Plasmid#205517PurposeNuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).DepositorInsertnuc-GsCT (GNAS Synthetic, Human)
UseTagsHistone H2A and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF5_P2A_Hygro_Barcode
Plasmid#120491PurposeBarcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF5 (KLF5 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR 2xU6:gRNA pten, mitfa:Cas9
Plasmid#118845PurposeTargets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorUseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB1
Plasmid#200981PurposeMammalian expression plasmid for myc-tagged EphB1DepositorInsertEphB1 (EPHB1 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp16
Plasmid#157724Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp16 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits