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Showing: 7041 - 7060 of 10528 results

  1. pENTR_L1-YFP-miniTurbo-L2

    Plasmid
    #127352
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127352-plasmid-map-sequence-id-246314

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  5. ZJOM43

    Plasmid
    #133652
    Purpose
    Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPS4-DuDre (VPS4 Budding Yeast)
    Use
    Tags
    DuDre
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133652-plasmid-map-sequence-id-308131

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  6. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

    Plasmid
    #127362
    Purpose
    gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127362-plasmid-map-sequence-id-246307

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  8. pSL3718 (pQCascade_Pse)

    Plasmid
    #200899
    Purpose
    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.
    Depositor
    Samuel H. Sternberg
    Article
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    PseCas7, PseCas8, PseCas6, PseTniQ
    Use
    Tags
    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200899-plasmid-map-sequence-id-393105

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  12. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  17. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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  19. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Expression
    Mutation
    Promoter
    RPS13 with Tet Operators Inserted
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59017-plasmid-map-sequence-id-423220

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Showing: 7041 - 7060 of 10528 results