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Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneUseTagsHIS-6-tag, PelB export signal, and Twin-Strep-tag…ExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only
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pAAV-CaMKII-STChRger2-TS-EYFP
Plasmid#129393PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Cytbow
Plasmid#158991PurposeMulticolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)DepositorInsertCytbow
UseTagsThe default expressed EBFP2 is fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mHRI-FLAG-IP
Plasmid#101791PurposeRetroviral vector encoding C-terminally FLAG tagged mouse HRIDepositorInsertHri (Eif2ak1 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR008_APAD
Plasmid#204818PurposeExpression Apobec1-ADAR2dd fusion protein as PIE-RBP controlDepositorInsertrat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
UseTagsHA and Flag and huADAR2ddExpressionMammalianMutationPromoterchicken β-actin promoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mPERK-FLAG-IP
Plasmid#101793PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PERKDepositorInsertPerk (Eif2ak3 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-MSK1
Plasmid#165601PurposeExpresses Sp dCas9 fused to human MSK1DepositorInsertribosomal protein S6 kinase A5 (RPS6KA5 S. Pyogenes, Human, Synthetic)
UseCRISPR and LentiviralTagsFLAG TagExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT GLI2 delta N
Plasmid#17649DepositorInsertGLI2 (GLI2 Human)
UseTags6x MycExpressionMammalianMutationMissing 328 N-terminal amino acidsPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His hTFNG
Plasmid#67240PurposeExpresses N-His tagged nonglycosylated human serum transferrin in mammalian cellsDepositorInserthuman serum transferrin (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn413 Asp, Asn611AspPromoterSV40Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor
Plasmid#184951PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM2-V5
Plasmid#180413Purposelentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) geneDepositorInsertTGM2 (TGM2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mPKR-FLAG-IG
Plasmid#101792PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PKRDepositorInsertPkr (Eif2ak2 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorInsertUPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-hAAT-IRES-ZsGreen-W
Plasmid#126702Purposelentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreenDepositorInsertAlpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM
Plasmid#169982PurposeTet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAMPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-H2B(1-114)_wt
Plasmid#118252PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNaDepositorInsertxlH2B(1-114)
UseTagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-EGFP
Plasmid#176274PurposeViral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK cyclin T1 (P#468)
Plasmid#14607DepositorInsertcyclin T1 (CCNT1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only