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pTOX10

Plasmid

#127455

Purpose

Encodes YhaV toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomCherry-Kan

Plasmid

#44903

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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Alpha 5 integrin-GFP

Plasmid

#15238

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

alpha 5 integrin (ITGA5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pCFD1-dU6:1gRNA

Plasmid

#49408

Purpose

expression of gRNA under control of the Drosophila U6:1 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-1:gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6-1

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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VSVG-mCherry-RUSH

Plasmid

#203647

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

VSVG

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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IP3 receptor, type 1 scFv [L24/21]

Plasmid

#206790

Purpose

Mammalian Expression of IP3 receptor, type 1 scFV. Derived from hybridoma L24/21 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IP3 receptor, type 1 (Rattus norvegicus) recombinant scFV (Itpr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pKm-HCBE

Plasmid

#170333

Purpose

a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized CBEs

Use

CRISPR

Tags

Expression

Mutation

Cas9n-D10A

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX33

Plasmid

#89632

Purpose

Allelic change mutagenesis

Depositor

Greg Cook

Article

Gebhard et al Microbiology. 2006 Nov;152(Pt 11):3453-6

Insert

xylE

Use

Allelic change mutagenesis

Tags

Expression

Mutation

Promoter

Pmyco

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-14-3-3 beta GST

Plasmid

#13276

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 beta (YWHAB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
HLA_A0101

Plasmid

#165009

Purpose

Expression of HLA-A*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-2mCh-PS

Plasmid

#112268

Purpose

IDOCKS Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

two copies of mCherry followed by a pseudosubstrate peptide for PKC

Use

Tags

mCh

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRuby4(1-10)

Plasmid

#165143

Purpose

Expresses mRuby4(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

mRuby4(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

Plasmid

#118593

Purpose

Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha/TRE3G

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pIRESneo-EGFP-alpha Tubulin

Plasmid

#12298

Depositor

Patricia Wadsworth

Article

Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

Insert

EGFP-alpha Tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE2

Plasmid

#197146

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE2 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERK-SPARK

Plasmid

#106921

Purpose

Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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opto-Tiam1

Plasmid

#174508

Purpose

Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

opto-Tiam1-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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5xNF-kb_RE::RedF

Plasmid

#124530

Purpose

NFKB RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

NF-κβ RedF reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-RPS27A-UTR-PP7-WPRE

Plasmid

#198339

Purpose

PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

RPS27A-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSVBneoT

Plasmid

#22883

Depositor

James Pipas

Article

Srinivasan et al Mol Cell Biol. 1997 Aug . 17(8):4761-73.

Insert

Early region SV40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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TRPM2_pCSdest

Plasmid

#53920

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TRPM2 (TRPM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-FLEX-GFP

Plasmid

#28304

Depositor

Edward Boyden

Insert

GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

Plasmid

#174007

Purpose

Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

Depositor

Mark Histed

Article

LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

Has Service

AAV9

Insert

jGCaMP8s-P2A-ChrimsonR-ST

Use

AAV

Tags

6xHis and soma targeting motif

Expression

Mutation

Promoter

hSyn

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAG32

Plasmid

#35122

Depositor

John McCusker

Article

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

hphMX4

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV.EF1a.ChR2-YFP.WPRE.hGH

Plasmid

#100056

Purpose

AAV expression of ChR2 fused to YFP driven byEF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Insert

ChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-Puro

Plasmid

#110841

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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PIP2-GFP(ST) sensor

Plasmid

#170356

Purpose

GFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

Depositor

Kees Jalink

Article

van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

Insert

GFP(ST)-PH (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-hAgo2

Plasmid

#21981

Depositor

Phil Sharp

Article

Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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PB-TAG-ERP2

Plasmid

#80479

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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