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Showing: 7021 - 7040 of 12975 results

  1. PB-TAC-Cas9

    Plasmid
    #120352
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on and CBh
    Availability
    Academic Institutions and Nonprofits only
    120352-plasmid-map-sequence-id-237238

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  2. PB-TAC-OKMS-Cas9

    Plasmid
    #120353
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120353-plasmid-map-sequence-id-236054

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  3. PB-TAC-OK+9MS-Cas9

    Plasmid
    #120354
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120354-plasmid-map-sequence-id-246101

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  4. PB-TAC-KRAB-dCas9

    Plasmid
    #120355
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on and CBh
    Availability
    Academic Institutions and Nonprofits only
    120355-plasmid-map-sequence-id-237262

    Enlarge

  5. PB-TAC-OKMS-KRAB-dCas9

    Plasmid
    #120356
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120356-plasmid-map-sequence-id-236039

    Enlarge

  6. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid
    #120357
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120357-plasmid-map-sequence-id-236025

    Enlarge

  15. pCRIS

    Plasmid
    #120424
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3, IS150
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    constitutive
    Availability
    Academic Institutions and Nonprofits only
    120424-plasmid-map-sequence-id-233246

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  16. pCRISP_IS

    Plasmid
    #120425
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    constitutive
    Availability
    Academic Institutions and Nonprofits only
    120425-plasmid-map-sequence-id-240993

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  18. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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Showing: 7021 - 7040 of 12975 results