Addgene

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pCu-Atg17(416) (I325D)

Plasmid

#121407

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

CUP1 promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgIdua.CMV/CB-EGFP

Plasmid

#121511

Purpose

Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgIdua

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgAspa.CMV/CB-EGFP

Plasmid

#121510

Purpose

Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgAspa

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pACEBAC1-CoREST-C

Plasmid

#109159

Purpose

Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD2 probe

Plasmid

#73070

Purpose

antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD2 (SNORD2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD60 probe

Plasmid

#73071

Purpose

antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD60 (SNORD60 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pHBS1292 TDP43 RRM-GFP G368W+W385G

Plasmid

#107838

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G368W and W385G mutations in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1039 TDP43 RRM-GFP 2xΦ

Plasmid

#107806

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All hydrophobics combined to two clusters in LLPS…

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1038 TDP43 RRM-GFP 4xΦ

Plasmid

#107805

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All hydrophobics combined to four clusters in LLP…

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1037 TDP43 RRM-GFP 6xΦ

Plasmid

#107804

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All hydrophobics combined to six clusters in LLPS…

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Bjoern Sander, Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Use

Tags

Twin-Strep tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Bjoern Sander, Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Use

Tags

Twin-Strep tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 N181-211 (#595)

Plasmid

#99939

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delAA181-211insNAAIRS linker

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 N187-207 (#596)

Plasmid

#99940

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delAA187-207insNAAIRS linker

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 1-209 (#430)

Plasmid

#99926

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-209

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 96-110 (#189)

Plasmid

#99929

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 96-110

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 111-135 (#190)

Plasmid

#99930

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 111-135

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 136-153 (#191)

Plasmid

#99931

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 136-153

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 200-204 (#597)

Plasmid

#99932

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 200-204

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 N132-145 (#433)

Plasmid

#99934

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delAA132-145insNAAIRS linker

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge