We narrowed to 9,533 results for: try

1. PAK4_HUMAN_D0

   Plasmid

   #79698

   Purpose

   This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PAK4 (PAK4 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. PAK6_HUMAN_D0

   Plasmid

   #79703

   Purpose

   This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PAK6 (PAK6 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pNC2

   Plasmid

   #72438

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg

   Promoter

   T7 and n/a

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK516

   Plasmid

   #71578

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamate-435 to glutamine

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK524

   Plasmid

   #71580

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes asparagine-347 to alanine

   Promoter

   T7

   Available Since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK513

   Plasmid

   #71576

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamate-294 to alanine

   Promoter

   T7

   Available Since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK564

   Plasmid

   #71581

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes serine-71 to alanine

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK517

   Plasmid

   #71579

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamate-435 to aspartate

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK514

   Plasmid

   #71577

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamine-435 to alanine

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK512

    Plasmid

    #71575

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes arginine-228 to glutamate

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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