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hSyn-Aion-citrine

Plasmid

#192569

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Article

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

Aion

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEA01

Plasmid

#170766

Purpose

Utilisation of pSAM2 excision tool in Amycolatopsis spp

Depositor

Jean-Luc Pernodet

Article

Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

Inserts

xis, int
oriT
pac

Use

Tags

Expression

Bacterial

Mutation

Promoter

trcp

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5D-FLAG

Plasmid

#72197

Purpose

Expresses full-length Unc5d with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d (Unc5d Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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LRRK2-K1906M

Plasmid

#35946

Depositor

Zhaoyang Feng

Article

Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

Insert

LRRK2 (LRRK2 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
LADL Anchor

Plasmid

#127664

Purpose

Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

dCas9-CIBN-2A-Puro

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 (D10A,H840A) mutant

Promoter

EF1a

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLCKO_LacZ_sgRNA

Plasmid

#74179

Purpose

lentiviral vector expressing sgRNA targeting LacZ

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

LacZ sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDHODH-4

Plasmid

#186022

Purpose

knock out DHODH in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGP18 (CK2alpha' K69M)

Plasmid

#27090

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' K69M (CSNK2A2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K69M

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

Plasmid

#182359

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Ultra-pan-neuronal positive control pUNP::gfp(NLS)

Use

Tags

Expression

Worm

Mutation

Promoter

Promoter Ultra Pan Neuronal

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAB07 (CK2beta)

Plasmid

#27091

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pLCKO_Luciferase_sgRNA

Plasmid

#74190

Purpose

lentiviral vector expressing sgRNA targeting Luciferase

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

Luciferase sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS R71/75E-HA

Plasmid

#130919

Purpose

Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E

Use

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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SHIP2 WT pBABE-His-Xpress

Plasmid

#126584

Purpose

Retroviral infection of MEF cells (puromycin selection)

Depositor

Christophe Erneux

Article

Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

Insert

SHIP2 (INPPL1 Human)

Use

Retroviral

Tags

His Xpress (N-terminal)

Expression

Mutation

Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pNS19-LV-mutRFP-2A-GFP

Plasmid

#113716

Purpose

Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

mutRFP-2A-GFP-IRES-Puro

Use

Lentiviral; Reporter system

Tags

Expression

Mammalian

Mutation

Y64L (mutRFP)

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV asyn S87A

Plasmid

#36056

Depositor

Hilal Lashuel

Article

Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

Insert

alpha-synuclein S87A (SNCA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

S87A

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

Plasmid

#124660

Purpose

reporter protein which emits bright blue fluorescence

Depositor

Yutaka Kuroda

Article

Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

Insert

Gaussia luciferase-C9D-TGinsert

Use

Tags

IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

Expression

Bacterial

Mutation

changed glutamic acid 100 to alanine, and glycine…

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ASAP2s

Plasmid

#101275

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Chamberland et al Elife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAMKIIa

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMD1_sgRNA_5

Plasmid

#74181

Purpose

lentiviral vector expressing sgRNA targeting PSMD1

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMD1 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

Plasmid

#184334

Purpose

Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

Nrxn3-alpha (Nrxn3 Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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