Addgene

Showing: 7001 - 7020 of 10528 results

Results per page:

Sort results by:

pLVX-Puro-TDP-43-WT

Plasmid

#133753

Purpose

Wild type human TDP-43 in pLVX-Puro lentiviral vector

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1-Tet-UPF1WT (pRKB264)

Plasmid

#99146

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1wt

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCCM’

Plasmid

#162709

Purpose

pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Use

Tags

Expression

Bacterial

Mutation

p15A origin replaced with high-copy colE1 origin

Promoter

PLtet0-1 promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB’

Plasmid

#162703

Purpose

pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Use

Tags

Expression

Bacterial

Mutation

5513T>G (tetR E37A); 7758G>T (second tet op…

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Use

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO mScarlet

Plasmid

#131002

Purpose

Double floxed mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

mScarlet

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-FAB(ER-LD)

Plasmid

#214420

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-FAB(mito-LD)

Plasmid

#214421

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-FAB(ER-mito)

Plasmid

#214423

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito

Use

Lentiviral

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MP9956:SFG.GD3synthase-2A-GD2synthase

Plasmid

#75013

Purpose

Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

Depositor

Martin Pule

Article

Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

Inserts

murine GD3 synthase
murine GD2 synthase

Use

Retroviral; Momlv retroviral vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FAB(PX-LD)

Plasmid

#214426

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FKBP-V5

Use

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N3-hARL1(WT)

Plasmid

#206406

Purpose

Mammalian expression of hARL1(WT)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

Plasmid

#198337

Purpose

PB plasmid expressing Halo-tagged PP7 coat protein

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

PP7 coat protein 3x Halo-GB1

Use

Piggybac

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_HNF4G_Hormone-recep

Plasmid

#109916

Purpose

Protein expression and purification of HNF4G_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HNF4G_Hormone-recep (HNF4G Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA Flag ERbeta

Plasmid

#35562

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-NPC1(P691S)-FLAG

Plasmid

#164973

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P691S

Promoter

EF1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge