Addgene

Showing: 7001 - 7020 of 21945 results

Results per page:

Sort results by:

Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

Plasmid

#208041

Purpose

Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

TurboID

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-Tet-OsTIR1(WT)-V5

Plasmid

#158663

Purpose

Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(WT) AAVS1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-OsTIR1(F74A)

Plasmid

#140731

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74A)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

Plasmid

#159689

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

Depositor

Dominic Esposito

Article

Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG/ATP9B-HA

Plasmid

#209214

Purpose

Mammalian expression of ATP9B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP9B (ATP9B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p39sheLL

Plasmid

#40627

Depositor

Simon Beddows

Article

Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

Inserts

HPV39 L1
HPV39 L2

Use

Tags

Expression

Mammalian

Mutation

Codon optimized for mammalian expression

Promoter

CMV and Polio IRES

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
CLXSN 5myc-CDC6-mut

Plasmid

#109333

Purpose

express tagged Cdc6 mutant

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

R56A, L59A, K81A, E82A, N83A

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Phi3-Zsgreen-PGK-mCherry

Plasmid

#153320

Purpose

Expresses zsGreen and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pInducer-YAP1-S127A

Plasmid

#213584

Purpose

Doxycycline inducible expression of YAP1-S127A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPI_SPbFGF

Plasmid

#25812

Depositor

Jozsef Kiss, Patrick Salmon

Article

Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

Insert

pWPI_SPbFGF (FGF2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Ig signal peptide in N-terminal of human FGF2

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECC-sgTom

Plasmid

#138661

Purpose

Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgTomato

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIH-Myc-YAP-del C

Plasmid

#33095

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

deleted C terminal (amino acids 291-450)

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
EF1a_TFAP2C_P2A_Hygro_Barcode

Plasmid

#120487

Purpose

Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

TFAP2C (TFAP2C Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
All-in-one lenti-BE4-NG

Plasmid

#200448

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Article

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19_CoV-2_linker

Plasmid

#211731

Purpose

Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Linker fragment SARS-CoV-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1 IntS11 AA 300-597

Plasmid

#199329

Purpose

Expresses His-tagged Drosophila IntS11 amino acids 300-597

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 11 (IntS11 Fly)

Use

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 300-597 only

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-ET

Plasmid

#72542

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa)

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMCK-T38S38-750-luxAB-DAS+4

Plasmid

#49518

Purpose

Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

T38S8-P750-luxAB-DAS+4

Use

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Mutation

Promoter

P750

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG671 mito-pFAST

Plasmid

#172867

Purpose

Expresses mitochondrial targeting domain-pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

mito-pFAST

Use

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge