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Plasmid#127456PurposeEncodes MqsR toxin. Gent-RDepositorInsertmqsR toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 GFP-NShroom3-iLID
Plasmid#170976PurposeN-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.DepositorInsertNShroom3 (Shroom3 Mouse)
UseGateway cloningTagseGFP and iLIDExpressionMutationFrom isoform 2, deleted amino acids 1389 - 1805,…PromoterAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 SspB-mCherry-CShroom3
Plasmid#170977PurposeC-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.DepositorInsertCShroom3 (Shroom3 Mouse)
UseGateway cloningTagsSspB and mCherryExpressionMutationIsoform X2, deleted aminoacids 1 - 1388PromoterAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ eiF4A1 myc HIS
Plasmid#71657Purposemammalian expression of eiF4A1DepositorInserteiF4A1 (EIF4A1 Human)
UseTagsHIS and mycExpressionMammalianMutationaa403 from Ala to ArgPromoterCMVAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG_GAPDH in pCW57_tTA_Blast
Plasmid#154211PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDHDepositorInsertGAPDH (GAPDH Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5F Smad2
Plasmid#12623DepositorInsertSmad2 (SMAD2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…ExpressionMutationPromoterRPS13 with Tet Operators InsertedAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
VEGFR2-YFP
Plasmid#108853PurposeEncodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorInsertKDR (KDR Human)
UseTagsLabeled with eYFP on the C-Terminus via a 3 amino…ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
paGFP-Omp25
Plasmid#69598Purposeexpresses GFP-labeled mitochondrial outer membrane protein 25DepositorInsertpaGFP-Omp25 (Synj2bp Rat)
UseLentiviralTagsGFPExpressionMammalianMutationphotoactivatable GFP fused to rat Omp25 C-terminusPromoterCMVAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC101-Donor
Plasmid#140630PurposeKanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.DepositorInsertLE, RE, KanR, temperature sensitive pSC101 origin of replication
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorUseTagsExpressionMammalianMutationPromoterMammalian Pol IAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGFP
Plasmid#32748DepositorInsertGFP
UseTagsDEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRLExpressionMammalianMutationS65TPromoterCMVAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
Plasmid#63729PurposeTo express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).UseTagsT2AExpressionMammalianMutationcodon optimizedPromoterSFFVAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-rABE-Flag
Plasmid#191383PurposeDoxycycline inducible expression of RNA A-to-I editor rABEDepositorInsertrABE-Flag
UseLentiviral and Synthetic BiologyTagsFlagExpressionMammalianMutationPromotertet-inducible promoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shBRCA1 #1
Plasmid#44594DepositorInsertBRCA1 (Brca1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-Sec61β
Plasmid#172442PurposeExpression of ER protein Sec61beta with an N-terminal FKBP-GFP tagDepositorInsertProtein transport protein Sec61 subunit beta
UseTagsFKBP-GFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFZ19
Plasmid#36184DepositorTypeEmpty backboneUsePlant t-dna vectorTagsExpressionPlantMutationPromoterXVEAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
synIRES_RC25
Plasmid#189970PurposePart 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAsDepositorInsertsynIRES_RC25
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1382
Plasmid#111436PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH2
Plasmid#129423PurposeExpression human MSH2 protein using baculovirusDepositorInserthuman MSH2 (MSH2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
LM627
Plasmid#191551PurposeLM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-hisDepositorInsertccdb
UseTagsSNAC-tag, HIS-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRBBm59
Plasmid#48115PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-Tn5
Plasmid#184286PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmKappa-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE60-EFTu-His
Plasmid#124127PurposeBacterial expression plasmid for PURE system componentsDepositorInsertElongation factor Tu (tufB Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max_3xHA
Plasmid#112096PurposeC:G-to-T:A base editingDepositorInsertBE4max_3xHA
UseCRISPRTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBWB419
Plasmid#140637PurposeRSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)
Plasmid#175573PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC
Plasmid#126218PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIaAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC414 - pAAV EF1a hPREP
Plasmid#59967PurposeAn AAV packaging vector that expresses hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPR219.05
Plasmid#78554PurposePlasmid expressing UirR, response regulator and PcsiR1-regulated GFP outputDepositorInsertuirR
UseSynthetic BiologyTagsExpressionMutationPromoterJ23115Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only