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Plasmid#195379PurposeMammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationIntron 18-runon PTPN22PromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Ex1-17-PTPN22
Plasmid#195375PurposeMammalian expression of a human PTPN22 mutant (exon 1-17) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationPTPN22 mutant containing exon 1 to 17PromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R3tdTomatoi2
Plasmid#185969PurposeExpresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.DepositorInsertPr-i2rbs-tdTomato cassette in reverse direction in Network Brick
UseSynthetic BiologyTagsExpressionMutationPromoterPRAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA/UIM/UBM-mut
Plasmid#187153PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationL2976A/P2977A/M1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUG-T2A-H93R
Plasmid#140739PurposeLentiviral vector to overexpress the autism-associated PTEN mutation H93R and GFP as two separate proteinsDepositorInsertGFP-T2A-PTENH93R (PTEN Human)
UseLentiviralTagsnoneExpressionMammalianMutationH93RPromoterhAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenN276S
Plasmid#140737PurposeLentiviral vector to overexpress the PTEN mutation N276S as a GFP-PTEN fusion proteinDepositorInsertPTEN – phosphatase and tensin homolog (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationN276SPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenW274L
Plasmid#140736PurposeLentiviral vector to overexpress the autism-associated PTEN mutation W274L as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationW274LPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenD252G
Plasmid#140735PurposeLentiviral vector to overexpress the autism-associated PTEN mutation D252G as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationD252GPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenH93R
Plasmid#140733PurposeLentiviral vector to overexpress the autism-associated PTEN mutation H93R as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationH93RPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only