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pRJPaph-bjGFP

Plasmid

#67032

Purpose

Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
bjGFP

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-sYFP2

Plasmid

#67033

Purpose

Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
sYFP2

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-mChe

Plasmid

#67034

Purpose

Plasmid for Paph-mCherry integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mCherry

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-HcRed

Plasmid

#67035

Purpose

Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
HcRed

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-gusA

Plasmid

#67036

Purpose

Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
gusA

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-lacZYA

Plasmid

#67037

Purpose

Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
lacZYA

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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TOPO pTRE

Plasmid

#68457

Purpose

TOPO construct expressing pTRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Tetracycline Response Element Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Availability

Academic Institutions and Nonprofits only

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pBT241_(pCA-ATG-intron-tTA2)

Plasmid

#36875

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tTA2

Use

Tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GIN-recipient

Plasmid

#11656

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP, IRES, and Neo

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTet-On-TAP

Plasmid

#59017

Purpose

Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.

Depositor

David Sibley

Article

Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

Type

Empty backbone

Use

Toxoplasma expression

Tags

Calmodulin Binding Peptide fused with a TEV Cleav…

Expression

Mutation

Promoter

RPS13 with Tet Operators Inserted

Availability

Academic Institutions and Nonprofits only

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pCS5

Plasmid

#55750

Purpose

Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Use

Tags

Expression

Bacterial

Mutation

Residues 59-264 of KanR

Promoter

T7-lac

Availability

Academic Institutions and Nonprofits only

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pCDNA5FRT/TO_HA-mAID-nanobody

Plasmid

#117713

Purpose

Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Use

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLD1 translation factors

Plasmid

#117760

Purpose

BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 1-13

Use

Escherichia coli

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/TO-myc-BirA*-NES

Plasmid

#112714

Purpose

For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

HIV Rev nuclear export signal (NES)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMRE160

Plasmid

#118508

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMRE161

Plasmid

#118509

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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