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pRK5-LYCHOS (GPR155) ∆DEP-HA

Plasmid

#199683

Purpose

Transient expression of LYCHOS (GPR155) ∆DEP-HA

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

DEP domain deleted

Promoter

CMV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 LYCHOS (GPR155) Y551A-HA

Plasmid

#199676

Purpose

Transient expression of LYCHOS (GPR155) Y551A mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y551 mutated to A

Promoter

CMV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) P44A-HA

Plasmid

#199675

Purpose

Transient expression of LYCHOS (GPR155) P44A mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P44 mutated to A

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) E48Q-HA

Plasmid

#199662

Purpose

Transient expression of LYCHOS (GPR155) E48Q mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E48 mutated to Q

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) F43I-HA

Plasmid

#199674

Purpose

Transient expression of LYCHOS (GPR155) F43I mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F43 mutated to I

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 PSK (745-1235)

Plasmid

#197863

Purpose

Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)

Depositor

Jonathan Morris

Article

Mitsopoulos et al J Biol Chem. 2003 May 16;278(20):18085-9

Insert

PSK1 (745-1235) (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

The N terminus of PSK1/TAOK2 has been deleted

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK1-beta

Plasmid

#197121

Purpose

Expression of MYC-tagged PSK1-beta in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

Insert

TAOK2 variant beta (aa1-1049) (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type variant beta

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2 (K57A)

Plasmid

#197118

Purpose

Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

Insert

TAOK1 (K57A) (TAOK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-delFS

Plasmid

#194555

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del FS" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Lys183

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-DVL1-MUT

Plasmid

#194587

Purpose

Mammalian Expression of mEGFP-DVL1 Mutant (H502Pfs*143) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

DVL1 (DVL1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

H502Pfs*143

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SOX2-MUT

Plasmid

#194581

Purpose

Mammalian Expression of mEGFP-SOX2 Mutant (M276Ifs*95) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SOX2 (SOX2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

M276Ifs*95

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-CALR-MUT

Plasmid

#194579

Purpose

Mammalian Expression of mEGFP-CALR Mutant (D387Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

CALR (CALR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

D387Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-PRKACG_IDG-K

Plasmid

#170603

Purpose

Gateway destination clone of PRKACG (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PRKACG (PRKACG Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFE-Rrub-FII-prk

Plasmid

#162695

Purpose

Expresses R. rubrum Form II rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Inserts

Rubrum Form II rubisco
S. elongatus PRK

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter and under same promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (D181N)

Plasmid

#112752

Purpose

express Flag-RagC (D181N mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (D181N) (RRAGC Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

181N

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-dSestrin (CG11299-PD)

Plasmid

#73675

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn (variant D) (Sesn Fly)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
R777-E192 Hs.PRKAA1-nostop

Plasmid

#70476

Purpose

Gateway ORF clone of human PRKAA1 [NM_006251.5] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

PRKAA1 (PRKAA1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E194 Hs.PRKAA2-nostop

Plasmid

#70478

Purpose

Gateway ORF clone of human PRKAA2 [NM_006252.3] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

PRKAA2 (PRKAA2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E202 Hs.PRKAG2-nostop

Plasmid

#70486

Purpose

Gateway ORF clone of human PRKAG2 [NM_024429.1] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

PRKAG2 (PRKAG2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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MR015-PRKAG2 Right TALEN

Plasmid

#49454

Purpose

Right TALEN for PRKAG2

Depositor

Bruce Conklin

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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