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pHH0103_RARA_Hormone-recep

Plasmid

#109836

Purpose

Protein expression and purification of RARA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RARA_Hormone-recep (RARA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLK1_POLO-box

Plasmid

#109812

Purpose

Protein expression and purification of PLK1_POLO-box

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLK1_POLO-box (PLK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPRED1_WH1

Plasmid

#109816

Purpose

Protein expression and purification of SPRED1_WH1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPRED1_WH1 (SPRED1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_TXN_Thioredoxin

Plasmid

#109778

Purpose

Protein expression and purification of TXN_Thioredoxin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TXN_Thioredoxin (TXN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).LC_v1

Plasmid

#133083

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).HC_v1

Plasmid

#133084

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Use

Tags

Expression

Bacterial

Mutation

wild-type C terminus

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Expression

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRLCon1288-1666

Plasmid

#31448

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon183-221_850-1207

Plasmid

#31449

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon183-221mut2_850-1207

Plasmid

#31450

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mut2 in balancer

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon1-449_850-1207

Plasmid

#31451

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCA14-pHR-SFFV-GFP-RPA43

Plasmid

#199445

Purpose

Expression of GFP-RPA43 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

RPA43 (POLR1F Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL9

Plasmid

#149520

Purpose

Express APEX2-Galectin-9 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS9 (LGALS9 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_WT

Plasmid

#111906

Purpose

Express RNASEH1 in mammalian cell.

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A2

Plasmid

#31100

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF4A (HNF4A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pdCASclos

Plasmid

#73640

Purpose

Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

dCas9
sgRNA to spo0A

Use

E.coli-clostridium shuttle vector

Tags

Expression

Mutation

D10A, H840A

Promoter

Pj23119 and ptb

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-TetO-PM

Plasmid

#197387

Purpose

To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral; Teto

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDarmo.CMVT_v1

Plasmid

#133072

Purpose

Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-Flag-TwinStrep and TwinStrep-Flag-3C

Expression

Mammalian

Mutation

Promoter

CMVT (Enhanced CMV)

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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