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Plasmid#120960PurposeMoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCerulean
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H96
Plasmid#170342PurposeCFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C91m
Plasmid#120999PurposeMoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertsfCFP (Chen, Bennett)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C92m
Plasmid#121000PurposeMoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).DepositorInsertsfCFPpdt3 (Chen,Bennett)
UseSynthetic BiologyTagspdt3 mfLon degradation tagExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C101m
Plasmid#121005PurposeMoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).DepositorInsertsfCFP with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H21
Plasmid#170335PurposeCFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(ST)_EPAC full length_Y(UST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H30
Plasmid#170337PurposeCFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H27
Plasmid#170336PurposeCFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(ST)_EPACdDEP _Y(UST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domainPromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZS2-123
Plasmid#26598DepositorInsertSynthetic
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNC1053
Plasmid#20406DepositorInsertCFP
UseTagsExpressionYeastMutationlinker: delta kPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC829
Plasmid#20386DepositorInsertCFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC860
Plasmid#20390DepositorInsertCFP
UseTagsExpressionYeastMutationLinker: ekPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
cdk1 FRET sensor (2327)
Plasmid#26064DepositorInsertCdk1 FRET Sensor
UseTagsCFP variant, FLAG, and YFP variantExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNC951
Plasmid#20400DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiY (see schematic) linker=deltakPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC953
Plasmid#20402DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiE (see schematic) linker=deltakPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC952
Plasmid#20401DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiM (see schematic) linker=deltakPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC861
Plasmid#20391DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiY (see schematic) linker: ekPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC955
Plasmid#20405DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiY (see schematic) linker=deltakPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC862
Plasmid#20392DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiM (see schematic) linker: ekPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC863
Plasmid#20393DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiE (see schematic) linker: ekPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only