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Plasmid#173235PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)
Plasmid#179080PurposeParent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminatorsDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-VP64-MCS_N-term
Plasmid#136886PurposeDoxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertVP64 activator domain
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-KRAB-MCS_N-term
Plasmid#136888PurposeDoxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertKRAB repressor domain
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM554 EF1α 3xFLAG-NLS-VP64-intN in TUPV2
Plasmid#161556PurposeConstitutive expression of 3xFLAG-NLS-VP64-intN under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM555 EF1α 3xFLAG-NLS-DsDed-intN in TUPV2
Plasmid#161557PurposeConstitutive expression of 3xFLAG-NLS-DsDed-intN under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM571 ZF2x6-C 3xFLAG-NLS-VP64-intN in TUPV2
Plasmid#161558PurposeInducible expression of 3xFLAG-NLS-VP64-intN under ZF2x6-C the promoterDepositorInsert3xFLAG-NLS-VP64-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF2x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM575 ZF6x6-C 3xFLAG-NLS-DsDed-ZF10 in TUPV2
Plasmid#161561PurposeInducible expression of 3xFLAG-NLS-DsDed-ZF10 under the ZF6x6-C promoterDepositorInsert3xFLAG-NLS-DsDed-ZF10
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM577 ZF6x6-C 3xFLAG-NLS-DsDed-intN in TUPV2
Plasmid#161563PurposeInducible expression of 3xFLAG-NLS-DsDed-intN under the ZF6x6-C promoterDepositorInsert3xFLAG-NLS-DsDed-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM595 ZF6x6-C intC-ZF1-ZF2-NLS-HA in TUPV2
Plasmid#161569PurposeInducible expression of intC-ZF1-ZF2-NLS-HA under the ZF6x6-C promoterDepositorInsertintC-ZF1-ZF2-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM583 EF1α 3xFLAG-NLS-VP64-ZF6 in TUPV2
Plasmid#161544PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF6 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF6
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM506 EF1α 3xFLAG-NLS-DsDed-ZF1 in TUPV2
Plasmid#161536PurposeConstitutive expression of 3xFLAG-NLS-DsDed-ZF1 gene under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM507 EF1α 3xFLAG-NLS-DsDed-ZF10 in TUPV2
Plasmid#161537PurposeConstitutive expression of 3xFLAG-NLS-DsDed-ZF10 under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-ZF10
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM512 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2
Plasmid#161538PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM520 EF1α 3xFLAG-NLS-VP64-ZF10 in TUPV4
Plasmid#161539PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF10 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF10
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM574 ZF2x6-C 3xFLAG-NLS-DsDed-ZF1 in TUPV2
Plasmid#161541PurposeInducible expression of 3xFLAG-NLS-DsDed-ZF1 under the ZF2x6-C promoterDepositorInsert3xFLAG-NLS-DsDed-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF2x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
Plasmid#159844PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(DEdel)-IDR-mCherry-CRY2-NLS
Plasmid#145273PurposeMammalian Expression of Nuclear Opto HOXD13(DEdel) IDR with SV40 NLSDepositorInsertHOXD13(D/E del)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationD/E deletedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(-15A)-IDR-mCherry-CRY2-NLS
Plasmid#145272PurposeMammalian Expression of Nuclear Opto HOXD13(-15A) IDR with SV40 NLSDepositorInsertHOXD13(-15A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat deletedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)
Plasmid#133798PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1(T114A/F115A)
UseTagsNLS(SV40)ExpressionMammalianMutationT114A/F115APromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSD32_pCAG-PV1-FKBP-L1-TP901-324C-NLS-BGHpA
Plasmid#108812PurposeExpresses aa324 to C-terminus of TP901 integrase with N-term FKBP and C-term NLSDepositorInsertTP901 integrase fragment aa324 to C-terminus
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2665_pCAG-PV1-FlpO-N27-L1-nMag-NLS-BGHpA
Plasmid#108825PurposeExpresses N-terminus to aa27 of Flp recombinase fused to nMag CIDDepositorInsertFlp recombinase fragment N-terminus to aa27
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2666_pCAG-PV1-pMag-L1-FlpO-28C-NLS-BGHpA
Plasmid#108826PurposeExpresses aa28 to C-terminus of Flp recombinase fused to pMag CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2669_pCAG-PV1-FlpO-N168-L1-nMag-NLS-BGHpA
Plasmid#108827PurposeExpresses N-terminus to aa168 of Flp recombinase fused to nMag CIDDepositorInsertFlp recombinase fragment N-terminus to aa168
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2670_pCAG-PV1-pMag-L1-FlpO-169C-NLS-BGHpA
Plasmid#108828PurposeExpresses aa169 to C-terminus of Flp recombinase fused to pMag CIDDepositorInsertFlp recombinase fragment aa169 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2673_pCAG-PV1-FlpO-N374-L1-nMag-NLS-BGHpA
Plasmid#108829PurposeExpresses N-terminus to aa374 of Flp recombinase fused to nMag CIDDepositorInsertFlp recombinase fragment N-terminus to aa374
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2674_pCAG-PV1-pMag-L1-FlpO-375C-NLS-BGHpA
Plasmid#108830PurposeExpresses aa375 to C-terminus of Flp recombinase fused to pMag CIDDepositorInsertFlp recombinase fragment aa375 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSD31_pCAG-PV1-TP901-N323-L1-FRB-NLS-BGHpA
Plasmid#108811PurposeExpresses N-terminus to aa323 of TP901 integrase fused to GID1 CIDDepositorInsertTP901 integrase fragment N-terminus to aa323
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2693_pCAG-PV1-iCre-N251-L1-nMag-NLS-BGHpA
Plasmid#108819PurposeExpresses N-terminus to aa251 of Cre recombinase fused to nMag CIDDepositorInsertCre recombinase fragment N-terminus to aa251
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2697_pCAG-PV1-iCre-N256-L1-nMag-NLS-BGHpA
Plasmid#108821PurposeExpresses N-terminus to aa256 of Cre recombinase fused to nMag CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1346_pCAG-PV1-PhiC31-N428-L1-FRB-NLS-BGHpA
Plasmid#108793PurposeExpresses N-terminus to aa428 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa428
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1359_pCAG-PV1-FKBP-L1-PhiC31-429C-NLS-BGHpA
Plasmid#108794PurposeExpresses aa429 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa429 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1601_pCAG-PV1-PhiC31-N396-L1-ABI-NLS-BGHpA
Plasmid#108799PurposeExpresses N-terminus to aa396 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1603_pCAG-PV1-PhiC31-N571-L1-ABI-NLS-BGHpA
Plasmid#108803PurposeExpresses N-terminus to aa571 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa571
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2586_pCAG-PV1-FKBP-L1-VCre-173C-NLS-BGHpA
Plasmid#108778PurposeExpresses aa173 to C-terminus of VCre recombinase fused to FKBP CIDDepositorInsertVCre recombinase fragment aa173 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2584_pCAG-PV1-VCre-N269-L1-FRB-NLS-BGHpA
Plasmid#108779PurposeExpresses N-terminus to aa269 of VCre recombinase fused to FRB CIDDepositorInsertVCre recombinase fragment N-terminus to aa269
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2588_pCAG-PV1-FKBP-L1-VCre-270C-NLS-BGHpA
Plasmid#108780PurposeExpresses aa270 to C-terminus of VCre recombinase fused to FKBP CIDDepositorInsertVCre recombinase fragment aa270 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2618_pCAG-PV1-VCre-N277-L1-FRB-NLS-BGHpA
Plasmid#108781PurposeExpresses N-terminus to aa277of VCre recombinase fused to FRB CIDDepositorInsertVCre recombinase fragment N-terminus to aa277
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2620_pCAG-PV1-FKBP-L1-VCre-278C-NLS-BGHpA
Plasmid#108782PurposeExpresses aa278 to C-terminus of VCre recombinase fused to FKBP CIDDepositorInsertVCre recombinase fragment aa278 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2619_pCAG-PV1-VCre-N277-L1-ABI-NLS-BGHpA
Plasmid#108787PurposeExpresses N-terminus to aa277of VCre recombinase fused to ABI CIDDepositorInsertVCre recombinase fragment N-terminus to aa277
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2621_pCAG-PV1-PYL-L1-VCre-278C-NLS-BGHpA
Plasmid#108788PurposeExpresses aa278 to C-terminus of VCre recombinase fused to PYL CIDDepositorInsertVCre recombinase fragment aa278 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1345_pCAG-PV1-PhiC31-N396-L1-FRB-NLS-BGHpA
Plasmid#108791PurposeExpresses N-terminus to aa396 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1358_pCAG-PV1-FKBP-L1-PhiC31-397C-NLS-BGHpA
Plasmid#108792PurposeExpresses aa397 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2274_pCAG-PV1-FlpO-N27-L1-ABI-NLS-BGHpA
Plasmid#108763PurposeExpresses N-terminus to aa27 of Flp recombinase fused to ABI CIDDepositorInsertFlp recombinase fragment N-terminus to aa27
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2275_pCAG-PV1-PYL-L1-FlpO-28C-NLS-BGHpA
Plasmid#108764PurposeExpresses aa28 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2282_pCAG-PV1-FlpO-N374-L1-ABI-NLS-BGHpA
Plasmid#108767PurposeExpresses N-terminus to aa374 of Flp recombinase fused to ABI CIDDepositorInsertFlp recombinase fragment N-terminus to aa374
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2283_pCAG-PV1-PYL-L1-FlpO-375C-NLS-BGHpA
Plasmid#108768PurposeExpresses aa375 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa375 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2550_pCAG-PV1-VCre-N277-L1-GID1-NLS-BGHpA
Plasmid#108775PurposeExpresses N-terminus to aa277of VCre recombinase fused to GID1 CIDDepositorInsertVCre recombinase fragment N-terminus to aa277
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2570_pCAG-PV1-GAI-L1-VCre-278C-NLS-BGHpA
Plasmid#108776PurposeExpresses aa278 to C-terminus of VCre recombinase fused to GAI CIDDepositorInsertVCre recombinase fragment aa278 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only