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Plasmid#192070PurposeExpression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.DepositorInsertsesRNA for tdTom
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry
Plasmid#36084Purpose3rd generation lentiviral plasmid expressing mCherryDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE191.pa
Plasmid#134931PurposeGEF-H1 biosensor that has GEF activity.DepositorInsertGEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
UseTagsNone.ExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-dTomato
Plasmid#99376PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-dTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
UseTags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC1-mitoRexYFP
Plasmid#60246PurposeMitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertmitoRexYFP
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max
Plasmid#112093PurposeC:G-to-T:A base editingDepositorInsertBE4max
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV GFP1-9
Plasmid#182244PurposeTripartite split-GFP mammalian. Express GFP1-9 detector fragmentDepositorInsertGFP 1-9 OPT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorInsertMLL-Af9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
FKBP-eGFP-TAZ
Plasmid#221213Purposepart A of TAZ-SPARK-OFFDepositorInsertFKBP-eGFP-TAZ
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB-cLUC
Plasmid#174051PurposeGateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assayDepositorTypeEmpty backboneUseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB-nLUC
Plasmid#174050PurposeGateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assayDepositorTypeEmpty backboneUseLuciferaseTagsN-terminal luciferaseExpressionPlantMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP
Plasmid#221233PurposeExpress sgRNA targeting HEK3 loci with nCas9(D10A)DepositorInsertSpCas9(D10A)-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W
Plasmid#67980PurposeCas9 activity reporter with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-hCD19
Plasmid#127889PurposeExpresses hCD19DepositorInserthCD19 (CD19 Human)
UseRetroviralTagsno tagsExpressionMutationnonePromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-BFP-sesRNA-GFP-WPRE-pA
Plasmid#192063PurposeExpression of BFP, sesRNA in mammalian cells, with GFP as efRNA.DepositorInsertsesRNA for tdTom
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xNFkB Luc
Plasmid#111216Purposereporter geneDepositorInsertfirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APEX2-nDEST
Plasmid#214992PurposeGateway compatible empty vector (lentiviral/mammalian expression) with an N-terminal APEX2 tagDepositorTypeEmpty backboneUseLentiviralTagsFlag-APEX2ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …PromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
DT960
Plasmid#80412PurposeExpresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pBpF
Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32G, E107P, D158T, I159SPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab11 WT
Plasmid#12674DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-HA
Plasmid#141320PurposePlasmid to carry out IVT of RfxCas13d (human codon-optimized)DepositorInsertRfx-Cas13d
UseCRISPRTagsHAExpressionMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgAAVS1
Plasmid#192477PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a controlDepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1
Plasmid#222337PurposePlasmid for expressing wild type Bxb1 in the mammalian cellsDepositorInsertCodon-optimized wild type Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
EB3-GFP
Plasmid#190164PurposeExpresses EB3-GFP in mammalian cellsDepositorInsertEB3-GFP
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 YFP
Plasmid#84910Purposeexpression of YFP in mammalian cellsDepositorInsertYFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-4mtD3cpv
Plasmid#36324DepositorInsert4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
UseTagsFirst 29 aa of human COX subunit 8aExpressionMutationPromoterCMVAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXPR_047
Plasmid#107145Purposemeasures Cas9 activityDepositorInsertGFP + sgRNA for GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0043-PspCas13b crRNA backbone
Plasmid#103854PurposeFor cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorHas ServiceCloning Grade DNAInsertU6-PspCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
moxGFP
Plasmid#68070PurposeC-terminal fusion to moxGFPDepositorTypeEmpty backboneUseTagsmoxGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HsPeg10rc3
Plasmid#174859PurposeExpresses recoded human Peg10 in mammalian cellsDepositorInsertrecoded Homo sapiens PEG10 (PEG10 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-BirA
Plasmid#20857DepositorInsertBirA
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDae079 (eMutaT7[transition])
Plasmid#187622PurposeExpresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertsTadA-8e-T7RNAP
PmCDA1-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only