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Addgene
Showing: 681 - 700 of 10428 results

  1. pNtCp-rbcL-accD

    Plasmid
    #52469
    Purpose
    Contains rbcL and accD DNA regions for double homologous recombination useful in Nicotiana tabacum Cp transformation
    Depositor
    Anastasios Melis
    Insert
    rbcL-accd Nicotiana tabacum Cp DNA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    52469-plasmid-map-sequence-id-81328

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  2. pNtCp-rbcL-PHLS-aadA-accD

    Plasmid
    #52470
    Purpose
    Inserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-aadA-accD
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    52470-plasmid-map-sequence-id-81333

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  3. pNtCp-rbcL-PHLS-GPPS-aadA-accD

    Plasmid
    #52471
    Purpose
    Inserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-GPPS-aadA-accD
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    52471-plasmid-map-sequence-id-81334

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  4. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid
    #74368
    Purpose
    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    yeGFP
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGRR-r11-r10
    Availability
    Academic Institutions and Nonprofits only
    74368-plasmid-map-sequence-id-141319

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  5. pMOD8A-iRGR-r11

    Plasmid
    #74369
    Purpose
    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r11
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AHD1
    Availability
    Academic Institutions and Nonprofits only
    74369-plasmid-map-sequence-id-141324

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  6. pMOD8A-RGR-r10

    Plasmid
    #74370
    Purpose
    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r10
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    74370-plasmid-map-sequence-id-141329

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  7. B0030m_BC

    Plasmid
    #74394
    Purpose
    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]
    Depositor
    Douglas Densmore
    Insert
    RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    74394-plasmid-map-sequence-id-140417

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  8. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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  9. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Availability
    Academic Institutions and Nonprofits only
    74495-plasmid-map-sequence-id-140495

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  10. pJM100: no promoter RFP on pCM66T

    Plasmid
    #74739
    Purpose
    pCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    74739-plasmid-map-sequence-id-146948

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  11. pJM102: pHps2 RFP on pCM66T

    Plasmid
    #74741
    Purpose
    pCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pHps2 (extracted from Methylovorus glucosetrophus…
    Availability
    Academic Institutions and Nonprofits only
    74741-plasmid-map-sequence-id-141458

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  12. pJM103: 150bp Hps2 RFP on pCM66T

    Plasmid
    #74744
    Purpose
    pCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pHps2, trimmed (extracted from Methylovorus gluco…
    Availability
    Academic Institutions and Nonprofits only
    74744-plasmid-map-sequence-id-141459

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  13. pJM105: pTac RFP on pCM66T

    Plasmid
    #74746
    Purpose
    pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTac
    Availability
    Academic Institutions and Nonprofits only
    74746-plasmid-map-sequence-id-146957

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  18. pJBL-RNAINS3

    Plasmid
    #69455
    Purpose
    RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S3
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    mutations for S3 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Availability
    Academic Institutions and Nonprofits only
    69455-plasmid-map-sequence-id-122579

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  19. pJBL-RNAINS4

    Plasmid
    #69456
    Purpose
    RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S4 + SFGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Availability
    Academic Institutions and Nonprofits only
    69456-plasmid-map-sequence-id-122578

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Showing: 681 - 700 of 10428 results