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Addgene
Showing: 681 - 700 of 11438 results
  1. pEGFPN1‐mTim1

    Plasmid
    #49215
    Purpose
    expresses EGFP tagged mouse Tim1
    Depositor
    Insert
    Tim1 (Havcr1 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. pmirGLO-Bmpr2-1719

    Plasmid
    #49379
    Purpose
    Insert is a fragment of the Bmpr2 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Insert
    Bmpr2 (BMPR2 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pmirGLO-Bmpr2-Δ17

    Plasmid
    #49380
    Purpose
    Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.
    Depositor
    Insert
    Bmpr2 (BMPR2 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    changed nucleotides AC to TG at bp 7347-7348
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pmirGLO-Bmpr2-Δ19

    Plasmid
    #49381
    Purpose
    Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-19 binding site. To be used for 3'UTR assay.
    Depositor
    Insert
    Bmpr2 (BMPR2 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    changed nucleotides TG to AC at bp 7344-7345
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pmirGLO-Smad5-1719

    Plasmid
    #49382
    Purpose
    Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Insert
    Smad5 (Smad5 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pmirGLO-Smad4-1719

    Plasmid
    #49383
    Purpose
    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Insert
    Smad family member 4 (SMAD4 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pLP-mOlig2-ires-gfp

    Plasmid
    #49399
    Purpose
    mammalian bicistronic expression of mouse Olig2 and EGFP
    Depositor
    Insert
    Olig2 (Olig2 Mouse)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP and IRES
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  8. MSCV-FlagmuEzh2deltaset-Hygro

    Plasmid
    #49403
    Purpose
    Expresses a Delta-set deletion mutant of Flag Tagged Ezh2. Hygromycin resistant.
    Depositor
    Insert
    3prime end of Flag-mus-Ezh2deltaSET, eliminating Set domain
    Use
    Retroviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Set domain deleted
    Promoter
    MSCV promotor
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid
    #50480
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    mCherry fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  12. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  13. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  14. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  15. pCDNA HA FAK P712/715A

    Plasmid
    #50502
    Depositor
    Insert
    protein tyrosine kinase, mutated
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    P712/715A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 681 - 700 of 11438 results