We narrowed to 15,889 results for: grna

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pCAG-Bmpr2ΔTail gRNA resistant

Plasmid

#163412

Purpose

Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The C-terminal tail domain (530-1038 aa) is delet…

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS2

Plasmid

#69234

Purpose

U6 driven SpCas9 sgRNA expression for TS2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS3

Plasmid

#69235

Purpose

U6 driven SpCas9 sgRNA expression for TS3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS4

Plasmid

#69236

Purpose

U6 driven SpCas9 sgRNA expression for TS4 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-KANK3

Plasmid

#69238

Purpose

U6 driven SpCas9 sgRNA expression for KANK3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

KANK3 sgRNA (KANK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-PLXNB2

Plasmid

#69240

Purpose

U6 driven SpCas9 sgRNA expression for PLXNB2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

PLXNB2 sgRNA (PLXNB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5T

Plasmid

#62264

Purpose

expression of A5T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5NT

Plasmid

#62263

Purpose

expression of A5NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A4T

Plasmid

#62262

Purpose

expression of A4T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A1NT

Plasmid

#62255

Purpose

expression of A1NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1NT

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-2

Plasmid

#161819

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgTh

Plasmid

#159901

Purpose

Mutagenesis of Th

Depositor

Larry Zweifel

Insert

Th (Th Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCP-tRNA

Plasmid

#133812

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter

Use

CRISPR

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_POU2F3_CR

Plasmid

#122175

Purpose

Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

Insert

POU2F3 (POU2F3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

3 silent mutations for gRNA resistance (882g-c, 8…

Promoter

EFS

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[3xPP7_SL]

Plasmid

#68424

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-shTRAF2#1

Plasmid

#44127

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral and RNAi

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pSMP-G9A_1

Plasmid

#36395

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD3_1

Plasmid

#36371

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD3 (MBD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER IKKe

Plasmid

#26211

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK epsilon (IKBKE Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only